Langen, Ralf and Chang, I-Jy and Germanas, Juris P. and Richards, John H. and Winkler, Jay R. and Gray, Harry B. (1995) Electron Tunneling in Proteins: Coupling Through a β Strand. Science, 268 (5218). pp. 1733-1735. ISSN 0036-8075. doi:10.1126/science.7792598. https://resolver.caltech.edu/CaltechAUTHORS:20150116-145929070
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150116-145929070
Abstract
Electron coupling through a beta strand has been investigated by measurement of the intramolecular electron-transfer (ET) rates in ruthenium-modified derivatives of the beta barrel blue copper protein Pseudomonas aeruginosa azurin. Surface histidines, introduced on the methionine-121 beta strand by mutagenesis, were modified with a Ru(2,2'-bipyridine)2(imidazole)^2+ complex. The Cu+ to Ru^3+ rate constants yielded a distance-decay constant of 1.1 per angstrom, a value close to the distance-decay constant of 1.0 per angstrom predicted for electron tunneling through an idealized beta strand. Activationless ET rate constants in combination with a tunneling-pathway analysis of the structures of azurin and cytochrome c confirm that there is a generally efficient network for coupling the internal (native) redox center to the surface of both proteins.
| Item Type: | Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| ORCID: |
| |||||||||
| Additional Information: | © 1995 American Association for the Advancement of Science. 17 January 1995; accepted 23 March 1995. We thank D. R. Casimiro, K. Warncke, J. J. Regan, and J. N. Onuchic for helpful comments. Supported by NIH and NSF. | |||||||||
| Funders: |
| |||||||||
| Issue or Number: | 5218 | |||||||||
| DOI: | 10.1126/science.7792598 | |||||||||
| Record Number: | CaltechAUTHORS:20150116-145929070 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150116-145929070 | |||||||||
| Official Citation: | Langen, R., Chang, I., Germanas, J., Richards, J., Winkler, & Gray, H. (1995). Electron tunneling in proteins: coupling through a beta strand. Science, 268(5218), 1733-1735. doi: 10.1126/science.7792598 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53841 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | INVALID USER | |||||||||
| Deposited On: | 17 Jan 2015 03:10 | |||||||||
| Last Modified: | 10 Nov 2021 20:07 |
Repository Staff Only: item control page
