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Assembly of Triple-Stranded β-Sheet Peptides at Interfaces

Rapaport, Hanna and Möller, Gunter and Knobler, Charles M. and Jensen, Torben R. and Kjaer, Kristian and Leiserowitz, Leslie and Tirrell, David A. (2002) Assembly of Triple-Stranded β-Sheet Peptides at Interfaces. Journal of the American Chemical Society, 124 (32). pp. 9342-9343. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20150116-153735804

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Abstract

A 30-residue peptide, BS30, which incorporates two proline residues to induce reverse turns, was designed to form a triple-stranded β-sheet monolayer at the air−water interface. To discern the structural role of proline, a second peptide, BS30G, identical to BS30 but with glycine residues replacing proline, was prepared and examined in parallel fashion. Surface pressure−molecular area isotherms indicated a limiting area per molecule (ca. 460 Å^2) for BS30 that corresponds well to that estimated from the known dimensions of crystalline β-sheet monolayers (492 Å^2). Comparable measurements on BS30G yielded a smaller molecular area (380 Å^2). Grazing incidence X-ray diffraction measurements performed on the BS30 monolayer at nominal area per molecule of 500 Å^2, exhibited two Bragg peaks corresponding to 4.79 and 34.9 Å spacings, consistent with formation of triple-stranded β-sheet structures that assemble into two-dimensional crystallites at the air−water interface. Visualized by Brewster angle microscopy, BS30 monolayers displayed uniform, solidlike domains, whereas BS30G appeared to be disordered.


Item Type:Article
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http://dx.doi.org/10.1021/ja026765jDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja026765jPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja026765jPublisherSupplemental material
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Alternate Title:Assembly of Triple-Stranded beta-Sheet Peptides at Interfaces
Additional Information:Copyright © 2002 American Chemical Society. Published In Issue August 14, 2002. Publication Date (Web): July 19, 2002. Received May 2, 2002. We thank Suzanna Horvath and her staff for peptide synthesis, George Rossman for help in ATR-FTIR, and HASYLAB for synchrotron beamtime. This work was supported by the U.S.-Israel Binational Science Foundation, the U.S. National Science Foundation, the DanSync program of the Danish Natural Science Research Council, and the European Community, TMR Contract ERBFMGECT950059. Supporting Information Available: Surface pressure-area isotherms, description of GIXD measurements, FTIR spectra, additional BAM images of BS30 (PDF).
Funders:
Funding AgencyGrant Number
U.S.-Israel Binational Science FoundationUNSPECIFIED
NSFUNSPECIFIED
DanSync Prgram (Danish Natural Science Research Council)UNSPECIFIED
European Community TMRERBFMGECT950059
Record Number:CaltechAUTHORS:20150116-153735804
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150116-153735804
Official Citation:Assembly of Triple-Stranded β-Sheet Peptides at Interfaces Hanna Rapaport, Gunter Möller, Charles M. Knobler, Torben R. Jensen, Kristian Kjaer, Leslie Leiserowitz, and David A. Tirrell Journal of the American Chemical Society 2002 124 (32), 9342-9343
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53847
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:16 Jan 2015 23:47
Last Modified:17 Jan 2015 02:55

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