Oka, Yuki and Ye, Mingyu and Zuker, Charles S. (2015) Thirst driving and suppressing signals encoded by distinct neural populations in the brain. Nature, 520 (7547). pp. 349-352. ISSN 0028-0836. PMCID PMC4401619. doi:10.1038/nature14108. https://resolver.caltech.edu/CaltechAUTHORS:20150120-093554854
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150120-093554854
Abstract
Thirst is the basic instinct to drink water. Previously, it was shown that neurons in several circumventricular organs of the hypothalamus are activated by thirst-inducing conditions. Here we identify two distinct, genetically separable neural populations in the subfornical organ that trigger or suppress thirst. We show that optogenetic activation of subfornical organ excitatory neurons, marked by the expression of the transcription factor ETV-1, evokes intense drinking behaviour, and does so even in fully water-satiated animals. The light-induced response is highly specific for water, immediate and strictly locked to the laser stimulus. In contrast, activation of a second population of subfornical organ neurons, marked by expression of the vesicular GABA transporter VGAT, drastically suppresses drinking, even in water-craving thirsty animals. These results reveal an innate brain circuit that can turn an animal’s water-drinking behaviour on and off, and probably functions as a centre for thirst control in the mammalian brain.
Item Type: | Article | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| ||||||||||||||||||||||||
Additional Information: | © 2015 Macmillan Publishers Limited. Received 02 September 2014; Accepted 24 November 2014; Published online 26 January 2015. We thank N. Propp for help with mouse husbandry. We also thank H. Fishman for suggestions, Z. Turan, N. Ryba and T. Usdin for technical support, and N. Ryba and members of the Zuker laboratory for comments. We acknowledge B. Lowell and M. Krashes for advice. Y.O. and M.Y. were supported by grants from the National Institute on Drug Abuse and National Institute of Neurological Disorders and Stroke to C.S.Z. C.S.Z. is an investigator of the Howard Hughes Medical Institute. Y.O. developed the research program, designed the study, carried out the experiments, and analysed data; M.Y. performed all slice patch clamp recordings; C.S.Z. analysed data, designed experiments and together with Y.O. wrote the paper. The authors declare no competing financial interests. | ||||||||||||||||||||||||
Funders: |
| ||||||||||||||||||||||||
Issue or Number: | 7547 | ||||||||||||||||||||||||
PubMed Central ID: | PMC4401619 | ||||||||||||||||||||||||
DOI: | 10.1038/nature14108 | ||||||||||||||||||||||||
Record Number: | CaltechAUTHORS:20150120-093554854 | ||||||||||||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150120-093554854 | ||||||||||||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||||||||||||
ID Code: | 53866 | ||||||||||||||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||||||||||||||
Deposited By: | George Porter | ||||||||||||||||||||||||
Deposited On: | 26 Jan 2015 17:28 | ||||||||||||||||||||||||
Last Modified: | 10 Nov 2021 20:08 |
Repository Staff Only: item control page