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Reassignment of sense codons in vivo

Link, A. James and Tirrell, David A. (2005) Reassignment of sense codons in vivo. Methods, 36 (3). pp. 291-298. ISSN 1046-2023. doi:10.1016/j.ymeth.2005.04.005.

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The genetic code maps one or more of the 61 sense codons onto a set of 20 canonical amino acids. Reassignment of sense codons to non-canonical amino acids in model organisms such as Escherichia coli has been achieved through manipulation of the cellular protein synthesis machinery. Specifically, control of amino acid pools, coupled with engineering of the aminoacyl-tRNA synthetase activity of the host, has enabled assignment of sense codons to a wide variety of non-canonical amino acids under conditions routinely used for expression of recombinant proteins. Codon reassignment is leading to important advances in protein engineering and bioorganic chemistry. Here we summarize some of those advances, and provide detailed protocols for codon reassignment.

Item Type:Article
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URLURL TypeDescription
Tirrell, David A.0000-0003-3175-4596
Additional Information:c2005 Elsevier Inc. Accepted 28 April 2005. Work on codon reassignment at Caltech has been supported by grants from the National Science Foundation (DMR 0080065), the National Institutes of Health (GM 62523), the U.S. Army Research Office (DAAD19-03-D-0004), and the Beckman Institute Endowment.
Funding AgencyGrant Number
NSFDMR 0080065
NIHGM 62523
Army Research Office (ARO)DAAD19-03-D-0004
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:Codon degeneracy; Non-canonical amino acids; Aminoacyl-tRNA synthetases; Aminoacylation
Issue or Number:3
Record Number:CaltechAUTHORS:20150120-160048550
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53901
Deposited By: Katherine Johnson
Deposited On:21 Jan 2015 00:07
Last Modified:10 Nov 2021 20:08

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