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Protein Folding Triggered by Electron Transfer

Pascher, Torbjörn and Chesick, John P. and Winkler, Jay R. and Gray, Harry B. (1996) Protein Folding Triggered by Electron Transfer. Science, 271 (5255). pp. 1558-1560. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150120-160536302

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Abstract

Rapid photochemical electron injection into unfolded ferricytochrome c titrated with 2.3 to 4.6 M guanidine hydrochloride (GuHCl) at pH 7 and 40°C produced unfolded ferrocytochrome, which then converted to the folded protein. Two folding phases were observed: a fast process with a time constant of 40 microseconds (4.6 M GuHCl), and a slower phase with a rate constant of 90 ± 20 per second (2.3 M GuHCl). The activation free energy for the slow step varied linearly with GuHCl concentration; the rate constant, extrapolated to aqueous solution, was 7600 per second. Electron-transfer methods can bridge the nanosecond to millisecond measurement time gap for protein folding.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.271.5255.1558DOIArticle
http://www.sciencemag.org/content/271/5255/1558PublisherArticle
http://www.jstor.org/stable/2890719JSTORArticle
ORCID:
AuthorORCID
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 1996 American Association for the Advancement of Science. 5 December 1995; Accepted 29 January 1996. We thank G. Mines and S. Lee for technical assistance, M. Gruebele for a preprint of his paper on apomyoglobin, and P. Wolynes for helpful discussions. Supported by the Swedish Natural Science Research Council (T.P.), NIH, NSF, and the Arnold and Mabel Beckman Foundation.
Funders:
Funding AgencyGrant Number
Swedish Natural Science Research CouncilUNSPECIFIED
NIHUNSPECIFIED
NSFUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20150120-160536302
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150120-160536302
Official Citation:Pascher, T., Chesick, J. P., Winkler, J. R., & Gray, H. B. (1996). Protein Folding Triggered by Electron Transfer. Science, 271(5255), 1558-1560. doi: 10.1126/science.271.5255.1558
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53902
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:21 Jan 2015 05:45
Last Modified:20 Sep 2017 23:26

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