Kiick, Kristi L. and van Hest, Jan C. M. and Tirrell, David A. (2000) Expanding the Scope of Protein Biosynthesis by Altering the Methionyl-tRNA Synthetase Activity of a Bacterial Expression Host. Angewandte Chemie International Edition, 39 (12). pp. 2148-2152. ISSN 1433-7851. doi:10.1002/1521-3773(20000616)39:12<2148::AID-ANIE2148>3.0.CO;2-7. https://resolver.caltech.edu/CaltechAUTHORS:20150121-143039773
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Abstract
The incorporation of amino acids into proteins in vivo is controlled by the aminoacyl-tRNA synthetases. The successful incorporation of a nonnatural amino acid, trans-crotylglycine (Tcg), into a protein has now been achieved by increasing the methionyl-tRNA synthetase activity of a bacterial expression host (see scheme). The incorporation of Tcg into proteins creates new opportunities for macromolecular synthesis through genetic engineering, due to the rich chemistry of the olefinic side chain.
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| Additional Information: | Issue published online: 14 JUN 2000. Article first published online: 14 JUN 2000. Manuscript Received: 26 JAN 2000. Scott Ross was helpful in conducting the 1D TOCSY NMR experiments and Pratip Bhattachary is thanked for assistance in other NMR experiments. We are grateful to Yves Mechulam for a sample of plasmid pBSM547W305F and to Hieronim Jakubowski of UMDNJ-New Jersey Medical School, Newark, New Jersey, for plasmid pGG3. K.L.K. thanks the U.S. Department of Defense for a National Defense Science and Engineering Graduate Fellowship. This work was supported by grants from the Polymers and Genetics Programs of the National Science Foundation and from the U.S. Army Research Office. | ||||||||
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| Subject Keywords: | amino acids; biosynthesis; ligases; protein expression; protein modifications | ||||||||
| Issue or Number: | 12 | ||||||||
| DOI: | 10.1002/1521-3773(20000616)39:12<2148::AID-ANIE2148>3.0.CO;2-7 | ||||||||
| Record Number: | CaltechAUTHORS:20150121-143039773 | ||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150121-143039773 | ||||||||
| Official Citation: | Kiick, K. L., van Hest, J. C. M. and Tirrell, D. A. (2000), Expanding the Scope of Protein Biosynthesis by Altering the Methionyl-tRNA Synthetase Activity of a Bacterial Expression Host . Angew. Chem. Int. Ed., 39: 2148–2152. doi: 10.1002/1521-3773(20000616)39:12<2148::AID-ANIE2148>3.0.CO;2-7 | ||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||
| ID Code: | 53956 | ||||||||
| Collection: | CaltechAUTHORS | ||||||||
| Deposited By: | Katherine Johnson | ||||||||
| Deposited On: | 21 Jan 2015 22:37 | ||||||||
| Last Modified: | 10 Nov 2021 20:25 |
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