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Expanding the Scope of Protein Biosynthesis by Altering the Methionyl-tRNA Synthetase Activity of a Bacterial Expression Host

Kiick, Kristi L. and van Hest, Jan C. M. and Tirrell, David A. (2000) Expanding the Scope of Protein Biosynthesis by Altering the Methionyl-tRNA Synthetase Activity of a Bacterial Expression Host. Angewandte Chemie International Edition, 39 (12). pp. 2148-2152. ISSN 1433-7851. https://resolver.caltech.edu/CaltechAUTHORS:20150121-143039773

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Abstract

The incorporation of amino acids into proteins in vivo is controlled by the aminoacyl-tRNA synthetases. The successful incorporation of a nonnatural amino acid, trans-crotylglycine (Tcg), into a protein has now been achieved by increasing the methionyl-tRNA synthetase activity of a bacterial expression host (see scheme). The incorporation of Tcg into proteins creates new opportunities for macromolecular synthesis through genetic engineering, due to the rich chemistry of the olefinic side chain.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/1521-3773(20000616)39:12<2148::AID-ANIE2148>3.0.CO;2-7DOIArticle
ORCID:
AuthorORCID
Kiick, Kristi L.0000-0001-8587-0301
Tirrell, David A.0000-0003-3175-4596
Additional Information:Issue published online: 14 JUN 2000. Article first published online: 14 JUN 2000. Manuscript Received: 26 JAN 2000. Scott Ross was helpful in conducting the 1D TOCSY NMR experiments and Pratip Bhattachary is thanked for assistance in other NMR experiments. We are grateful to Yves Mechulam for a sample of plasmid pBSM547W305F and to Hieronim Jakubowski of UMDNJ-New Jersey Medical School, Newark, New Jersey, for plasmid pGG3. K.L.K. thanks the U.S. Department of Defense for a National Defense Science and Engineering Graduate Fellowship. This work was supported by grants from the Polymers and Genetics Programs of the National Science Foundation and from the U.S. Army Research Office.
Funders:
Funding AgencyGrant Number
National Defense Science and Engineering Graduate (NDSEG) FellowshipUNSPECIFIED
NSFUNSPECIFIED
Army Research Office (ARO)UNSPECIFIED
Subject Keywords: amino acids; biosynthesis; ligases; protein expression; protein modifications
Issue or Number:12
Record Number:CaltechAUTHORS:20150121-143039773
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150121-143039773
Official Citation:Kiick, K. L., van Hest, J. C. M. and Tirrell, D. A. (2000), Expanding the Scope of Protein Biosynthesis by Altering the Methionyl-tRNA Synthetase Activity of a Bacterial Expression Host . Angew. Chem. Int. Ed., 39: 2148–2152. doi: 10.1002/1521-3773(20000616)39:12<2148::AID-ANIE2148>3.0.CO;2-7
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53956
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:21 Jan 2015 22:37
Last Modified:28 Feb 2020 00:22

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