CaltechAUTHORS
  A Caltech Library Service

Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability

Tang, Yi and Ghirlanda, Giovanna and Petka, Wendy A. and Nakajima, Tadashi and DeGrado, William F. and Tirrell, David A. (2001) Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability. Angewandte Chemie International Edition, 40 (8). pp. 1494-1496. ISSN 1433-7851. https://resolver.caltech.edu/CaltechAUTHORS:20150121-143801079

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150121-143801079

Abstract

Fluorination of the hydrophobic core of a coiled-coil protein significantly improved its stability toward thermal and chemical denaturation. 5′,5′,5′-Trifluoroleucine (2) was efficiently incorporated into a leucine-zipper protein in place of leucine (1) during E. coli biosynthesis. The fluorinated variant maintained stable secondary and tertiary structures under conditions that caused denaturation of the “wild-type” protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/1521-3773(20010417)40:8<1494::AID-ANIE1494>3.0.CO;2-XDOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/1521-3773%2820010417%2940:8%3C1494::AID-ANIE1494%3E3.0.CO;2-X/abstractPublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Issue published online: 17 APR 2001. Article first published online: 17 APR 2001. Manuscript Received: 2 JAN 2001. This work was supported by a grant from the U.S. Army Research Office. Y. Tang is supported by a Whitaker Graduate Research Fellowship. We thank Dr. Gary Hathaway for performing matrix-assisted laser desorption/ionization analyses.
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)UNSPECIFIED
Whitaker Graduate Research FellowshipUNSPECIFIED
Subject Keywords:biosynthesis; circular dichroism; fluorine; helical structures; protein structures
Issue or Number:8
Record Number:CaltechAUTHORS:20150121-143801079
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150121-143801079
Official Citation:Tang, Y., Ghirlanda, G., Petka, W. A., Nakajima, T., DeGrado, W. F. and Tirrell, D. A. (2001), Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability . Angew. Chem. Int. Ed., 40: 1494–1496. doi: 10.1002/1521-3773(20010417)40:8<1494::AID-ANIE1494>3.0.CO;2-X
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53958
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:21 Jan 2015 22:43
Last Modified:03 Oct 2019 07:54

Repository Staff Only: item control page