Tang, Yi and Ghirlanda, Giovanna and Petka, Wendy A. and Nakajima, Tadashi and DeGrado, William F. and Tirrell, David A. (2001) Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability. Angewandte Chemie International Edition, 40 (8). pp. 1494-1496. ISSN 1433-7851. doi:10.1002/1521-3773(20010417)40:8<1494::AID-ANIE1494>3.0.CO;2-X. https://resolver.caltech.edu/CaltechAUTHORS:20150121-143801079
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Abstract
Fluorination of the hydrophobic core of a coiled-coil protein significantly improved its stability toward thermal and chemical denaturation. 5′,5′,5′-Trifluoroleucine (2) was efficiently incorporated into a leucine-zipper protein in place of leucine (1) during E. coli biosynthesis. The fluorinated variant maintained stable secondary and tertiary structures under conditions that caused denaturation of the “wild-type” protein.
| Item Type: | Article | |||||||||
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| Additional Information: | Issue published online: 17 APR 2001. Article first published online: 17 APR 2001. Manuscript Received: 2 JAN 2001. This work was supported by a grant from the U.S. Army Research Office. Y. Tang is supported by a Whitaker Graduate Research Fellowship. We thank Dr. Gary Hathaway for performing matrix-assisted laser desorption/ionization analyses. | |||||||||
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| Subject Keywords: | biosynthesis; circular dichroism; fluorine; helical structures; protein structures | |||||||||
| Issue or Number: | 8 | |||||||||
| DOI: | 10.1002/1521-3773(20010417)40:8<1494::AID-ANIE1494>3.0.CO;2-X | |||||||||
| Record Number: | CaltechAUTHORS:20150121-143801079 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150121-143801079 | |||||||||
| Official Citation: | Tang, Y., Ghirlanda, G., Petka, W. A., Nakajima, T., DeGrado, W. F. and Tirrell, D. A. (2001), Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability . Angew. Chem. Int. Ed., 40: 1494–1496. doi: 10.1002/1521-3773(20010417)40:8<1494::AID-ANIE1494>3.0.CO;2-X | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53958 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Katherine Johnson | |||||||||
| Deposited On: | 21 Jan 2015 22:43 | |||||||||
| Last Modified: | 10 Nov 2021 20:25 |
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