Alternative Translations of a Single RNA Message: An Identity Switch of (2S,3R)-4,4,4-Trifluorovaline between Valine and Isoleucine Codons

Wang, Pin and Fichera, Alfio and Kumar, Krishna and Tirrell, David A. (2004) Alternative Translations of a Single RNA Message: An Identity Switch of (2S,3R)-4,4,4-Trifluorovaline between Valine and Isoleucine Codons. Angewandte Chemie International Edition, 43 (28). pp. 3664-3666. ISSN 1433-7851. doi:10.1002/anie.200454036. https://resolver.caltech.edu/CaltechAUTHORS:20150121-144351506

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Abstract

Changed in translation: Bacterial expression hosts can be engineered so that a single RNA message can be read in different ways depending on the relative rates of competing aminoacylation reactions. The (2S,3R)-4,4,4-trifluorovaline can be assigned either to isoleucine or to valine codons according to whether the bacterial host overexpresses the isoleucyl- or the valyl-tRNA synthetase (IleRS and ValRS, respectively; see scheme).

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1002/anie.200454036	DOI	Article
http://onlinelibrary.wiley.com/doi/10.1002/anie.200454036/abstract	Publisher	Article
http://onlinelibrary.wiley.com/doi/10.1002/anie.200454036/suppinfo	Publisher	Supplemental material

ORCID:

Author	ORCID
Kumar, Krishna	0000-0001-5318-4622
Tirrell, David A.	0000-0003-3175-4596

Additional Information:

© 2004 Wiley VCH. Issue published online: 7 JUL 2004. Article first published online: 21 JUN 2004. Manuscript Received: 17 FEB 2004. This work was supported by the National Institutes of Health (grant nos. GM65500 and GM62523), by a National Sciences Foundation CAREER award (CHE-0236846), and by the National Science Foundation Center for Science and Engineering of Materials at the California Institute of Technology.

Funders:

Funding Agency	Grant Number
NIH	GM65500
NIH	GM62523
NSF	CHE-0236846

Subject Keywords:

amino acids; biosynthesis; gene expression; tRNA

Issue or Number:

DOI:

10.1002/anie.200454036

Record Number:

CaltechAUTHORS:20150121-144351506

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20150121-144351506

Official Citation:

Wang, P., Fichera, A., Kumar, K. and Tirrell, D. A. (2004), Alternative Translations of a Single RNA Message: An Identity Switch of (2S,3R)-4,4,4-Trifluorovaline between Valine and Isoleucine Codons. Angew. Chem. Int. Ed., 43: 3664–3666. doi: 10.1002/anie.200454036

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ID Code:

53960

Collection:

CaltechAUTHORS

Deposited By:

Katherine Johnson

Deposited On:

21 Jan 2015 22:51

Last Modified:

10 Nov 2021 20:25

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