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Kinetics of membrane micellization by the hydrophobic polyelectrolyte poly( 2-ethylacrylic acid)

Thomas, James L. and Devlin, Brian P. and Tirrell, David A. (1996) Kinetics of membrane micellization by the hydrophobic polyelectrolyte poly( 2-ethylacrylic acid). Biochimica et Biophysica Acta - Biomembranes, 1278 (1). pp. 73-78. ISSN 0005-2736. https://resolver.caltech.edu/CaltechAUTHORS:20150122-100001276

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Abstract

Rates of pH-dependent micellization of multilamellar vesicles by the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) (PEAA) have been measured turbidometrically. This polymer shows a strong pH-dependence in its affinity for phospholipid membranes, binding in increasing amounts as pH is lowered and ultimately solubilizing membranes to form mixed micelles (Tirrell, Takigawa and Seki (1985) Ann. N.Y. Acad. Sci. 446, 237). The rate of solubilization of dipalmitoylphosphatidylcholine (DPPC) vesicle suspensions by PEAA increases approximately linearly with reductions in pH below a threshold at pH 6.55. Interestingly, negatively-charged dipalmitoylphosphatidylglycerol membranes showed qualitatively similar behavior in the presence of PEAA, and incorporation of 10% or 20% dipalmitoylphosphatidic acid in DPPC membranes did not affect solubilization rates, demonstrating that membrane charge is not an important factor in determining micellization kinetics. Micellization of DPPC and dimyrstoylphosphatidylcholine membranes occurs most rapidly at their respective gel-liquid crystalline transition temperatures (Tm); the rate enhancement is correlated with a peak in the temperature-dependent binding of a fluorescently-modified PEAA in slightly alkaline solutions in which no micellization is observed. The lateral compressibility of the membrane, which has a similar peak at T_m, is proposed to be an important determinant of the rate and extent of polymer adsorption, and consequently of the rate of micellization.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0005-2736(95)00192-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0005273695001921PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 1996 Published by Elsevier B.V. Received 7 April 1995; accepted 17 July 1995. Acknowledgements: This work was supported by the National Science Foundation Materials Research Laboratory and the Center for Environmentally Appropriate Materials at the University of Massachusetts.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Center for Environmentally Appropriate Materials, University of MassachusettsUNSPECIFIED
Subject Keywords: Phosphatidylcholine; Phosphatidylglycerol; Polyelectrolyte; Micellization; Adsorption; Kinetics
Issue or Number:1
Record Number:CaltechAUTHORS:20150122-100001276
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150122-100001276
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53987
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:22 Jan 2015 18:08
Last Modified:03 Oct 2019 07:54

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