Chen, Chun-Nan and Denome, Sylvia and Davis, Ronald L. (1986) Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce' gene, the structural gene for cAMP phosphodiesterase. Proceedings of the National Academy of Sciences of the United States of America, 83 (24). pp. 9313-9317. ISSN 0027-8424. doi:10.1073/pnas.83.24.9313. https://resolver.caltech.edu/CaltechAUTHORS:20150126-155115174
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Abstract
We have isolated and sequenced cDNA clones representing portions of the polyadenylylated transcripts of the dunce+ gene. These define an open reading frame of 1086 bases and some of the 5'- and 3'-untranslated regions of the transcripts. The deduced amino acid sequence is strikingly homologous to the amino acid sequence of a Ca^2+/calmodulin-dependent cyclic nucleotide phosphodiesterase isolated from bovine brain and more weakly related to the predicted amino acid sequence of a yeast cAMP phosphodiesterase. These homologies, together with prior genetic and biochemical studies, provide unambiguous evidence that dunce^+ codes for a phosphodiesterase. In addition, the dunce^+ gene product shares a seven-amino acid sequence with a regulatory subunit of cAMP-dependent protein kinase that is predicted to be part of the cAMP binding site. We also identify a weak homology between a region of the dunce+ gene product and the egg-laying hormone precursor of Aplysia californica. The open reading frame is divided in the genome by four introns.
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| Additional Information: | © 1986 National Academy of Sciences. Communicated by Norman Davidson, August 21, 1986. We thank those who have provided cDNA libraries for our use; R. Scheller and R. Axel for helpful discussions concerning ELH; and the J. Beavo, J. Kiger, and M. Wigler laboratories for sharing their results prior to publication. This work was supported by National Institutes of Health Grant NS-19904, a Research Career Development Award, and a McKnight Scholars Award to R.L.D. | |||||||||
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| Issue or Number: | 24 | |||||||||
| DOI: | 10.1073/pnas.83.24.9313 | |||||||||
| Record Number: | CaltechAUTHORS:20150126-155115174 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150126-155115174 | |||||||||
| Official Citation: | Chen, C. N., Denome, S., & Davis, R. L. (1986). Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase. Proceedings of the National Academy of Sciences, 83(24), 9313-9317. | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 54098 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Jason Perez | |||||||||
| Deposited On: | 27 Jan 2015 00:26 | |||||||||
| Last Modified: | 10 Nov 2021 20:29 |
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