Chen, Chun-Nan and Denome, Sylvia and Davis, Ronald L. (1986) Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase. Proceedings of the National Academy of Sciences of the United States of America, 83 (24). pp. 9313-9317. ISSN 0027-8424. PMCID PMC387128. doi:10.1073/pnas.83.24.9313. https://resolver.caltech.edu/CaltechAUTHORS:20150126-155115174
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Abstract
We have isolated and sequenced cDNA clones representing portions of the polyadenylylated transcripts of the dunce+ gene. These define an open reading frame of 1086 bases and some of the 5'- and 3'-untranslated regions of the transcripts. The deduced amino acid sequence is strikingly homologous to the amino acid sequence of a Ca²⁺/calmodulin-dependent cyclic nucleotide phosphodiesterase isolated from bovine brain and more weakly related to the predicted amino acid sequence of a yeast cAMP phosphodiesterase. These homologies, together with prior genetic and biochemical studies, provide unambiguous evidence that dunce+ codes for a phosphodiesterase. In addition, the dunce+ gene product shares a seven-amino acid sequence with a regulatory subunit of cAMP-dependent protein kinase that is predicted to be part of the cAMP binding site. We also identify a weak homology between a region of the dunce+ gene product and the egg-laying hormone precursor of Aplysia californica. The open reading frame is divided in the genome by four introns.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1986 National Academy of Sciences. Communicated by Norman Davidson, August 21, 1986. We thank those who have provided cDNA libraries for our use; R. Scheller and R. Axel for helpful discussions concerning ELH; and the J. Beavo, J. Kiger, and M. Wigler laboratories for sharing their results prior to publication. This work was supported by National Institutes of Health Grant NS-19904, a Research Career Development Award, and a McKnight Scholars Award to R.L.D. | |||||||||
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| Issue or Number: | 24 | |||||||||
| PubMed Central ID: | PMC387128 | |||||||||
| DOI: | 10.1073/pnas.83.24.9313 | |||||||||
| Record Number: | CaltechAUTHORS:20150126-155115174 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150126-155115174 | |||||||||
| Official Citation: | Chen, C. N., Denome, S., & Davis, R. L. (1986). Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase. Proceedings of the National Academy of Sciences, 83(24), 9313-9317. | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 54098 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | INVALID USER | |||||||||
| Deposited On: | 27 Jan 2015 00:26 | |||||||||
| Last Modified: | 06 Jun 2023 18:23 |
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