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Design of Sequence-Specific DNA-Binding Molecules

Dervan, Peter B. (1986) Design of Sequence-Specific DNA-Binding Molecules. Science, 232 (4749). pp. 464-471. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150127-105928628

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Abstract

Base sequence information can be stored in the local structure of right-handed double-helical DNA (B-DNA). The question arises as to whether a set of rules for the three-dimensional readout of the B-DNA helix can be developed. This would allow the design of synthetic molecules that bind DNA of any specific sequence and site size. There are four stages of development for each new synthetic sequence-specific DNA-binding molecule: design, synthesis, testing for sequence specificity, and reevaluation of the design. This approach has produced bis(distamycin)fumaramide, a synthetic, crescent-shaped oligopeptide that binds nine contiguous adenine-thymine base pairs in the minor groove of double-helical DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.2421408DOIArticle
http://www.sciencemag.org/content/232/4749/464PublisherArticle
http://www.jstor.org/stable/1696414JSTORArticle
Additional Information:© 1986 American Association for the Advancement of Science. Supported by NIH grant GM-27681, American Cancer Society grant NP-428, and the Burroughs Wellcome Company.
Funders:
Funding AgencyGrant Number
NIHGM-27681
American Cancer SocietyNP-428
Burroughs Wellcome UNSPECIFIED
Record Number:CaltechAUTHORS:20150127-105928628
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150127-105928628
Official Citation:Design of sequence-specific DNA-binding molecules PB Dervan Science 25 April 1986: 232 (4749), 464-471. [DOI:10.1126/science.2421408]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54139
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Jan 2015 06:50
Last Modified:28 Jan 2015 06:50

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