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Assays for Cholinergic Properties in Cultured Rat Schwann Cells

Brockes, J. P. (1984) Assays for Cholinergic Properties in Cultured Rat Schwann Cells. Proceedings of the Royal Society of London. Series B, Biological Sciences, 222 (1226). pp. 121-134. ISSN 0962-8452. doi:10.1098/rspb.1984.0053.

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Cultured rat Schwann cells did not contain detectable levels of choline acetyltransferase (less than 0.5 pmol ACh min^(-1) mg^(-1) of protein) or of acetylcholinesterase and nicotinic acetylcholine receptors. After adding Schwann cells to primary rat myotube cultures, the level of cholineacetyl transferase in the co-cultures increased after three weeks to as high as 5 pmol ACh min^(-1) mg^(-1) . The activity appearing in co-cultures sedimented at approximately 4S, and was inhibited 50% by 4(1-napthylvinyl) pyridine in the concentration range of 10-50 μM. After treatment of co-cultures with anti rat neural antigen-1 (RAN-1) and complement, 70-80% of the activity was lost, suggesting that it is induced in the Schwann cells. Attempts to obtain the effect by exposure of Schwann cells to medium conditioned by the myotube cultures, or by co-culture with fibroblasts gave levels of activity at or below the limit of detection.

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Additional Information:© 1984 The Royal Society. Communicated by B. B. Boycott, F.R.S. Received 28 February 1984. Initial experiments on expression of cholinergic properties were performed at the M.R.C. Neuroimmunology Project, University College London and I thank M. C. Raff, J. Winter and A. Hornby-Smith for their participation. Research at the California Institute of Technology was supported by grants from the N.I.H. and the Pew Memorial Trust. I thank T. Stevens for assistance with cultures and Dr K. L. Fields for the gift of anti-RAN-1.
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Pew Memorial TrustUNSPECIFIED
Issue or Number:1226
Record Number:CaltechAUTHORS:20150127-135027104
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Official Citation:Assays for Cholinergic Properties in Cultured Rat Schwann Cells J. P. Brockes Proceedings of the Royal Society of London. Series B, Biological Sciences Vol. 222, No. 1226 (Jul. 23, 1984) , pp. 121-134 Published by: The Royal Society Stable URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54150
Deposited By: Ruth Sustaita
Deposited On:28 Jan 2015 00:48
Last Modified:10 Nov 2021 20:29

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