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Zinc-Dependent Structure of a Single-Finger Domain of Yeast ADR1

Párraga, Grace and Horvath, Suzanna J. and Eisen, Arri and Taylor, Wayne E. and Hood, Leroy and Young, Elton T. and Klevit, Rachel E. (1988) Zinc-Dependent Structure of a Single-Finger Domain of Yeast ADR1. Science, 241 (4872). pp. 1489-1492. ISSN 0036-8075. doi:10.1126/science.3047872.

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In the proposed "zinc finger" DNA-binding motif, each repeat unit binds a zinc metal ion through invariant Cys and His residues and this drives the folding of each 30-residue unit into an independent nucleic acid-binding domain. To obtain structural information, we synthesized single and double zinc finger peptides from the yeast transcription activator ADR1, and assessed the metal-binding and DNA-binding properties of these peptides, as well as the solution structure of the metal-stabilized domains, with the use of a variety of spectroscopic techniques. A single zinc finger can exist as an independent structure sufficient for zinc-dependent DNA binding. An experimentally determined model of the single finger is proposed that is consistent with circular dichroism, one- and two-dimensional nuclear magnetic resonance, and visual spectroscopy of the single-finger peptide reconstituted in the presence of zinc.

Item Type:Article
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URLURL TypeDescription DOIArticle
Hood, Leroy0000-0001-7158-3678
Additional Information:© 1988 American Association for the Advancement of Science. 15 June 1988; accepted 12 August 1988. We thank B. R. Reid and G. Drobny and their groups for use of NMR facilities; H. Charbonneau, S. Kumar, M. Harrylock, R. Wade, and K. Walsh for assistance with amino acid compositional analysis and for sequencing the peptide; and our colleagues D. Allison, J. Herriott, and B. M. Shapiro for their helpful comments on the manuscript.
Issue or Number:4872
Record Number:CaltechAUTHORS:20150128-094117616
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Official Citation:Parraga, G., Horvath, S., Eisen, A., Taylor, W., Hood, L., Young, E., & Klevit, R. (1988). Zinc-dependent structure of a single-finger domain of yeast ADR1. Science, 241(4872), 1489-1492. doi: 10.1126/science.3047872
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54171
Deposited By: Jason Perez
Deposited On:28 Jan 2015 23:21
Last Modified:10 Nov 2021 20:30

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