Párraga, Grace and Horvath, Suzanna J. and Eisen, Arri and Taylor, Wayne E. and Hood, Leroy and Young, Elton T. and Klevit, Rachel E. (1988) Zinc-Dependent Structure of a Single-Finger Domain of Yeast ADR1. Science, 241 (4872). pp. 1489-1492. ISSN 0036-8075. doi:10.1126/science.3047872. https://resolver.caltech.edu/CaltechAUTHORS:20150128-094117616
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Abstract
In the proposed "zinc finger" DNA-binding motif, each repeat unit binds a zinc metal ion through invariant Cys and His residues and this drives the folding of each 30-residue unit into an independent nucleic acid-binding domain. To obtain structural information, we synthesized single and double zinc finger peptides from the yeast transcription activator ADR1, and assessed the metal-binding and DNA-binding properties of these peptides, as well as the solution structure of the metal-stabilized domains, with the use of a variety of spectroscopic techniques. A single zinc finger can exist as an independent structure sufficient for zinc-dependent DNA binding. An experimentally determined model of the single finger is proposed that is consistent with circular dichroism, one- and two-dimensional nuclear magnetic resonance, and visual spectroscopy of the single-finger peptide reconstituted in the presence of zinc.
Item Type: | Article | ||||||||||||
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Additional Information: | © 1988 American Association for the Advancement of Science. 15 June 1988; accepted 12 August 1988. We thank B. R. Reid and G. Drobny and their groups for use of NMR facilities; H. Charbonneau, S. Kumar, M. Harrylock, R. Wade, and K. Walsh for assistance with amino acid compositional analysis and for sequencing the peptide; and our colleagues D. Allison, J. Herriott, and B. M. Shapiro for their helpful comments on the manuscript. | ||||||||||||
Issue or Number: | 4872 | ||||||||||||
DOI: | 10.1126/science.3047872 | ||||||||||||
Record Number: | CaltechAUTHORS:20150128-094117616 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150128-094117616 | ||||||||||||
Official Citation: | Parraga, G., Horvath, S., Eisen, A., Taylor, W., Hood, L., Young, E., & Klevit, R. (1988). Zinc-dependent structure of a single-finger domain of yeast ADR1. Science, 241(4872), 1489-1492. doi: 10.1126/science.3047872 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 54171 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Jason Perez | ||||||||||||
Deposited On: | 28 Jan 2015 23:21 | ||||||||||||
Last Modified: | 10 Nov 2021 20:30 |
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