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URF6, last unidentified reading frame of human mtDNA, codes for an NADH dehydrogenase subunit

Chomyn, Anne and Cleeter, Michael W. J. and Ragan, C. Ian and Riley, Marcia and Doolittle, Russell F. and Attardi, Giuseppe (1986) URF6, last unidentified reading frame of human mtDNA, codes for an NADH dehydrogenase subunit. Science, 234 (4776). pp. 614-618. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150130-124807876

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Abstract

The polypeptide encoded in URF6, the last unassigned reading frame of human mitochondrial DNA, has been identified with antibodies to peptides predicted from the DNA sequence. Antibodies prepared against highly purified respiratory chain NADH dehydrogenase from beef heart or against the cytoplasmically synthesized 49-kilodalton iron-sulfur subunit isolated from this enzyme complex, when added to a deoxycholate or a Triton X-100 mitochondrial lysate of HeLa cells, specifically precipitated the URF6 product together with the six other URF products previously identified as subunits of NADH dehydrogenase. These results strongly point to the URF6 product as being another subunit of this enzyme complex. Thus, almost 60% of the protein coding capacity of mammalian mitochondrial DNA is utilized for the assembly of the first enzyme complex of the respiratory chain. The absence of such information in yeast mitochondrial DNA dramatizes the variability in gene content of different mitochondrial genomes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.3764430DOIArticle
http://www.sciencemag.org/content/234/4776/614PublisherArticle
http://www.jstor.org/stable/1697865JSTORArticle
Additional Information:© 1986 American Association for the Advancement of Science. 15 May 1986; Accepted2 6 August 1986. We thank D. Brow and D. Trovato for assistance in the synthesis of the peptides and P. Mariottini for help in the early immunoprecipitation experiments. Supported by NIH grant GM-11726 to G.A., a grant from the Science and Engineering Research Council to C.I.R., and NSF grant PCM-8118172 to R.F.D. We thank A. Drew for technical help.
Funders:
Funding AgencyGrant Number
NIHGM-11726
Science and Engineering Research Council (UK)UNSPECIFIED
NSFPCM-8118172
Record Number:CaltechAUTHORS:20150130-124807876
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150130-124807876
Official Citation:Chomyn, A., Cleeter, M., Ragan, C., Riley, M., Doolittle, R., & Attardi, G. (1986). URF6, last unidentified reading frame of human mtDNA, codes for an NADH dehydrogenase subunit. Science, 234(4776), 614-618. doi: 10.1126/science.3764430
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54248
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:31 Jan 2015 23:20
Last Modified:31 Jan 2015 23:20

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