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Detection of c-sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells

Graves, D. T. and Owen, A. J. and Barth, R. K. and Tempst, P. and Winoto, A. and Fors, L. and Hood, L. E. and Antoniades, H. N. (1984) Detection of c-sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells. Science, 226 (4677). pp. 972-974. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20150130-153529829

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Abstract

Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.6209798DOIArticle
http://www.sciencemag.org/content/226/4677/972PublisherArticle
http://www.jstor.org/stable/1693369JSTORArticle
Additional Information:© 1984 American Association for the Advancement of Science. 18 May 1984; Accepted 27 July 1984. We thank S. Williams, R. Siraco, G. Easterly, C. Katz, and J. MacLaren for technical assistance. Supported by National Cancer Institute grant CA30101 (H.N.A.) and NIH grants HL29583 (H.N.A.) and HL27607 (A.J.O.). R.K.B. is a fellow of the Anna Fuller Fund and P.T. is a special fellow of the Leukemia Society of America.
Funders:
Funding AgencyGrant Number
National Cancer InstituteCA30101
NIHHL29583
NIHHL27607
Anna Fuller FundUNSPECIFIED
Leukemia Society of AmericaUNSPECIFIED
Issue or Number:4677
Record Number:CaltechAUTHORS:20150130-153529829
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150130-153529829
Official Citation:Graves, D., Owen, A., Barth, R., Tempst, P., Winoto, A., Fors, L., . . . Antoniades, H. (1984). Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells. Science, 226(4677), 972-974. doi: 10.1126/science.6209798
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54262
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:03 Feb 2015 23:31
Last Modified:03 Oct 2019 07:56

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