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DNA Charge Transport within the Cell

Grodick, Michael A. and Muren, Natalie B. and Barton, Jacqueline K. (2015) DNA Charge Transport within the Cell. Biochemistry, 54 (4). pp. 962-973. ISSN 0006-2960. PMCID PMC4587570. http://resolver.caltech.edu/CaltechAUTHORS:20150202-150621170

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Abstract

The unique characteristics of DNA charge transport (CT) have prompted an examination of roles for this chemistry within a biological context. Not only can DNA CT facilitate long-range oxidative damage of DNA, but redox-active proteins can couple to the DNA base stack and participate in long-range redox reactions using DNA CT. DNA transcription factors with redox-active moieties such as SoxR and p53 can use DNA CT as a form of redox sensing. DNA CT chemistry also provides a means to monitor the integrity of the DNA, given the sensitivity of DNA CT to perturbations in base stacking as arise with mismatches and lesions. Enzymes that utilize this chemistry include an interesting and ever-growing class of DNA-processing enzymes involved in DNA repair, replication, and transcription that have been found to contain 4Fe-4S clusters. DNA repair enzymes containing 4Fe-4S clusters, that include endonuclease III (EndoIII), MutY, and DinG from bacteria, as well as XPD from archaea, have been shown to be redox-active when bound to DNA, share a DNA-bound redox potential, and can be reduced and oxidized at long-range via DNA CT. Interactions between DNA and these proteins in solution, in addition to genetics experiments within Escherichia coli, suggest that DNA-mediated CT can be used as a means of cooperative signaling among DNA repair proteins that contain 4Fe-4S clusters as a first step in finding DNA damage, even within cells. On the basis of these data, we can consider also how DNA-mediated CT may be used as a means of signaling to coordinate DNA processing across the genome.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi501520wDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi501520wPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587570PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2015 American Chemical Society. Received: December 15, 2014; Revised: January 14, 2015; Publication Date (Web): January 21, 2015. Funding: Research funded by the Center for Chemical Signaling through the Gordon and Betty Moore Foundation, and the National Institutes of Health (GM61077). We are grateful to our many co-workers and collaborators for their diligence and creativity in exploring this chemistry. The authors declare no competing financial interest.
Group:Caltech Center for Environmental Microbial Interactions (CEMI)
Funders:
Funding AgencyGrant Number
Gordon and Betty Moore FoundationUNSPECIFIED
NIHGM61077
PubMed Central ID:PMC4587570
Record Number:CaltechAUTHORS:20150202-150621170
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150202-150621170
Official Citation:DNA Charge Transport within the Cell Michael A. Grodick, Natalie B. Muren, and Jacqueline K. Barton Biochemistry 2015 54 (4), 962-973 DOI: 10.1021/bi501520w
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54309
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Feb 2015 04:06
Last Modified:20 Oct 2017 22:47

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