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Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation

King, Michael P. and Attardi, Giuseppe (1989) Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science, 246 (4929). pp. 500-503. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150203-135431077

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Abstract

Two human cell lines (termed ρ^0), which had been completely depleted of mitochondrial DNA (mtDNA) by long-term exposure to ethidium bromide, were found to be dependent on uridine and pyruvate for growth because of the absence of a functional respiratory chain. Loss of either of these two metabolic requirements was used as a selectable marker for the repopulation of ρ^0 cells with exogenous mitochondria by complementation. Transformants obtained with various mitochondrial donors exhibited a respiratory phenotype that was in most cases distinct from that of the ρ^0 parent or the donor, indicating that the genotypes of the mitochondrial and nuclear genomes as well as their specific interactions play a role in the respiratory competence of a cell.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.2814477 DOIArticle
http://www.sciencemag.org/content/246/4929/500PublisherArticle
http://www.jstor.org/stable/1704589 JSTORArticle
Additional Information:© 1989 American Association for the Advancement of Science. 24 April 1989; accepted 28 August 1989. Supported by NIH grant GM11726. We thank S. Lai for carrying out the cytochrome c oxidase assays, B. Kceley for determining growth curves, P. C. Gaines of the Caltech Microchemical Facility for the extraction of DNA samples, J. Houldsworth and our colleagues in the laboratory for reading the manuscript, and A. Drew and L. Tefo for technical help.
Funders:
Funding AgencyGrant Number
NIHGM11726
Record Number:CaltechAUTHORS:20150203-135431077
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150203-135431077
Official Citation:King, M., & Attardi, G. (1989). Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science, 246(4929), 500-503. doi: 10.1126/science.2814477
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54332
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:03 Feb 2015 22:37
Last Modified:03 Feb 2015 22:37

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