CaltechAUTHORS
  A Caltech Library Service

Recognition of Thymine Adenine Base Pairs by Guanine in a Pyrimidine Triple Helix Motif

Griffin, Linda C. and Dervan, Peter B. (1989) Recognition of Thymine Adenine Base Pairs by Guanine in a Pyrimidine Triple Helix Motif. Science, 245 (4921). pp. 967-971. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150204-145754564

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20150204-145754564

Abstract

Oligonucleotide recognition offers a powerful chemical approach for the sequence-specific binding of double-helical DNA. In the pyrimidine-Hoogsteen model, a binding size of >15 homopurine base pairs affords >30 discrete sequence-specific hydrogen bonds to duplex DNA. Because pyrimidine oligonucleotides limit triple helix formation to homopurine tracts, it is desirable to determine whether oligonucleotides can be used to bind all four base pairs of DNA. A general solution would allow targeting of oligonucleotides (or their analogs) to any given sequence in the human genome. A study of 20 base triplets reveals that the triple helix can be extended from homopurine to mixed sequences. Guanine contained within a pyrimidine oligonucleotide specifically recognizes thymine.adenine base pairs in duplex DNA. Such specificity allows binding at mixed sites in DNA from simian virus 40 and human immunodeficiency virus.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.2549639 DOIArticle
http://www.sciencemag.org/content/245/4921/967PublisherArticle
http://www.jstor.org/stable/1704195JSTORArticle
Additional Information:© 1989 American Association for the Advancement of Science. 26 April 1989; accepted 7 July 1989. We thank the National Institutes of Health (GM-35724), the Office of Naval Research, and the Parsons Foundation for generous support. We thank S. Singleton for valuable assistance in model building, A. Frankel for a gift of pHIV-CAT, and T. Povsic for large-scale plasmid preparation.
Funders:
Funding AgencyGrant Number
NIHGM-35724
Office of Naval Research (ONR)UNSPECIFIED
Parsons FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20150204-145754564
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150204-145754564
Official Citation:Griffin, L., & Dervan, P. (1989). Recognition of thymine adenine.base pairs by guanine in a pyrimidine triple helix motif. Science, 245(4921), 967-971. doi: 10.1126/science.2549639
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54386
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:05 Feb 2015 04:50
Last Modified:05 Feb 2015 04:51

Repository Staff Only: item control page