CaltechAUTHORS
  A Caltech Library Service

Identification of a BALB/c H-2L^d gene by DNA-mediated gene transfer

Goodenow, Robert S. and McMillan, Minnie and Örn, Anders and Nicolson, Margery O. and Davidson, Norman and Frelinger, Jeffrey A. and Hood, Leroy (1982) Identification of a BALB/c H-2L^d gene by DNA-mediated gene transfer. Science, 215 (4533). pp. 677-679. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150204-152008392

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20150204-152008392

Abstract

Gene transfer and immunoselection were used in the identification of a BALB/c genomic clone containing an H-2L^d gene (clone 27.5). Transformation of thymidine kinase-negative C3H mouse L cells with the cloned 27.5 DNA together with the herpes simplex virus tk gene produced transformants expressing L^d molecules detected by radioimmune assay with monoclonal hybridoma antibodies to Ld antigens. The foreign L^d gene products expressed by cloned mouse L cell transformants were shown to be virtually indistinguishable from BALB/c spleen L^d molecules by two-dimensional electrophoretic analysis of H-2L^d immunoprecipitates. These results indicate that the genomic clone 27.5 contains a functional BALB/c H-2L^d gene and demonstrate the usefulness of this approach for identifying the gene products encoded by cloned genes which are members of a multigene family. Furthermore, the ability to place cell-surface recognition molecules on the surfaces of foreign cells provides a powerful opportunity for functional analyses of these molecules.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.7058331DOIArticle
http://www.sciencemag.org/content/215/4533/677.abstractPublisherArticle
http://www.jstor.org/stable/1688211JSTORArticle
Additional Information:© 1982 American Association for the Advancement of Science. 5 November 1981; revised 16 December 1981. Supported by NIH grants GM 06965, CA 22662, CA 26199, and CA 25911. R.S.G. is a senior Lievre fellow of the California Division of the American Cancer Society. J .A.F. is the recipient of an American Cancer Society faculty research award. We thank D. Sachs and T. Hansen for supplying the monoclonal hybridoma antibodies used in these studies.
Funders:
Funding AgencyGrant Number
NIHGM 06965
NIHCA 22662
NIHCA 26199
NIHCA 25911
Record Number:CaltechAUTHORS:20150204-152008392
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150204-152008392
Official Citation:Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer RS Goodenow, M McMillan, A Orn, M Nicolson, N Davidson, JA Frelinger, and L Hood Science 5 February 1982: 215 (4533), 677-679. [DOI:10.1126/science.7058331]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54390
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Feb 2015 04:56
Last Modified:05 Feb 2015 04:56

Repository Staff Only: item control page