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Identification of a BALB/c H-2L^d gene by DNA-mediated gene transfer

Goodenow, Robert S. and McMillan, Minnie and Örn, Anders and Nicolson, Margery O. and Davidson, Norman and Frelinger, Jeffrey A. and Hood, Leroy (1982) Identification of a BALB/c H-2L^d gene by DNA-mediated gene transfer. Science, 215 (4533). pp. 677-679. ISSN 0036-8075. doi:10.1126/science.7058331.

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Gene transfer and immunoselection were used in the identification of a BALB/c genomic clone containing an H-2L^d gene (clone 27.5). Transformation of thymidine kinase-negative C3H mouse L cells with the cloned 27.5 DNA together with the herpes simplex virus tk gene produced transformants expressing L^d molecules detected by radioimmune assay with monoclonal hybridoma antibodies to Ld antigens. The foreign L^d gene products expressed by cloned mouse L cell transformants were shown to be virtually indistinguishable from BALB/c spleen L^d molecules by two-dimensional electrophoretic analysis of H-2L^d immunoprecipitates. These results indicate that the genomic clone 27.5 contains a functional BALB/c H-2L^d gene and demonstrate the usefulness of this approach for identifying the gene products encoded by cloned genes which are members of a multigene family. Furthermore, the ability to place cell-surface recognition molecules on the surfaces of foreign cells provides a powerful opportunity for functional analyses of these molecules.

Item Type:Article
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Hood, Leroy0000-0001-7158-3678
Additional Information:© 1982 American Association for the Advancement of Science. 5 November 1981; revised 16 December 1981. Supported by NIH grants GM 06965, CA 22662, CA 26199, and CA 25911. R.S.G. is a senior Lievre fellow of the California Division of the American Cancer Society. J .A.F. is the recipient of an American Cancer Society faculty research award. We thank D. Sachs and T. Hansen for supplying the monoclonal hybridoma antibodies used in these studies.
Funding AgencyGrant Number
NIHGM 06965
NIHCA 22662
NIHCA 26199
NIHCA 25911
Issue or Number:4533
Record Number:CaltechAUTHORS:20150204-152008392
Persistent URL:
Official Citation:Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer RS Goodenow, M McMillan, A Orn, M Nicolson, N Davidson, JA Frelinger, and L Hood Science 5 February 1982: 215 (4533), 677-679. [DOI:10.1126/science.7058331]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54390
Deposited By: Ruth Sustaita
Deposited On:05 Feb 2015 04:56
Last Modified:10 Nov 2021 20:32

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