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DNA Sequence of the Gene Encoding the E_ɑ Ia Polypeptide of the BALB/c Mouse

McNicholas, Janet and Steinmetz, Michael and Hunkapiller, Tim and Jones, Patricia and Hood, Leroy (1982) DNA Sequence of the Gene Encoding the E_ɑ Ia Polypeptide of the BALB/c Mouse. Science, 218 (4578). pp. 1229-1232. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150205-071548043

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Abstract

A 3.4-kilobase DNA fragment containing the gene coding for the Eɑ chain of an Ia (I region-associated) antigen from the BALB/c mouse has been sequenced. It contains at least three exons, which correlate with the major structural domains of the Eɑ chain-the two external domains al and ɑ2, and the transmembrane-cytoplasmic domain. The coding sequence of the mouse Eɑ gene shows striking homology to its human counterpart at the DNA and protein levels. The translated ɑ2 exon demonstrates significant similarity to β_2-microglohulin, to immunoglobulin constant region domains, and to certain domains of transplantation antigens. These observations and those of others suggest that the Ia antigen, transplantation antigen, and immunoglohulin gene families share a common ancestor.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.6815800 DOIArticle
http://www.sciencemag.org/content/218/4578/1229.abstractPublisherArticle
http://www.jstor.org/stable/1689000JSTORArticle
Additional Information:© 1982 American Association for the Advancement of Science. 23 August 1982; revised 20 September 1982. Supported by grants from the National Institutes of Health. We thank D. Fisher for his guidance throughout this work, especially for help with the deoxyribonuclease deletion subcloning and sequencing strategy. We thank A. Korman and J. Strominger, D. Larhammar, and P. Peterson for making the class II DNA sequences and preprints available to us prior to publication and for helpful discussions. We also thank K. Minard for preparation of sequencing gels and B. Larsh for preparation of the manuscript. J.M. is a postdoctoral fellow of the Arthritis Foundation. M.S. was supported by a Senior Lievre fellowship from the California Division of the American Cancer Society.
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Arthritis FoundationUNSPECIFIED
California Division of the American Cancer Society Senior Lievre fellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20150205-071548043
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150205-071548043
Official Citation:DNA sequence of the gene encoding the E alpha Ia polypeptide of the BALB/c mouse J McNicholas, M Steinmetz, T Hunkapiller, P Jones, and L Hood Science 17 December 1982: 218 (4578), 1229-1232. [DOI:10.1126/science.6815800]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54395
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Feb 2015 17:26
Last Modified:05 Feb 2015 17:26

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