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Nitrogenase Iron-Molybdenum Cofactor Binding Site: Protein Conformational Changes Associated with Cofactor Binding

Magnuson, Jon K. and Paustian, Timothy D. and Shah, Vinod K. and Dean, Dennis R. and Roberts, Gary P. and Rees, Douglas C. and Howard, James B. (1997) Nitrogenase Iron-Molybdenum Cofactor Binding Site: Protein Conformational Changes Associated with Cofactor Binding. Tetrahedron, 53 (35). pp. 11971-11984. ISSN 0040-4020. https://resolver.caltech.edu/CaltechAUTHORS:20150205-140103112

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Abstract

Formation of active nitrogenase MoFe-protein requires the assembly and insertion of a unique molybdenum containing Fe:S cluster, the FeMoco. The protein acceptor is held in an open conformation by the binding of a small molecular weight protein, γ. Using selective alkylation of the protein cysteines, conformational changes associated with γ binding and cofactor insertion were evaluated and a model for the change presented. Reversible oxygen damage to the precursor is identified and evaluated in terms of the chemistry of dithionite used in the buffer as an oxygen scavenger.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0040-4020(97)00710-2DOIArticle
http://www.sciencedirect.com/science/article/pii/S0040402097007102PublisherArticle
ORCID:
AuthorORCID
Rees, Douglas C.0000-0003-4073-1185
Contact Email Address:phoebe@caltech.edu
Additional Information:© 1997 Elsevier Science Ltd. Received 10 April 1997; accepted 12 June 1997. This work was supported by NSF grants MCB-9513512 (JBH), MCB-9317059 (GR), MCB-9630127 (DD) and DMB 91-18689.
Funders:
Funding AgencyGrant Number
NSFMCB-9513512
NSFMCB-9317059
NSFMCB-9630127
NSFDMB 91-18689
Issue or Number:35
Record Number:CaltechAUTHORS:20150205-140103112
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150205-140103112
Official Citation:Jon K. Magnuson, Timothy D. Paustian, Vinod K. Shah, Dennis R. Dean, Gary P. Roberts, Douglas C. Rees, James B. Howard, Nitrogenase iron-molybdenum cofactor binding site: Protein conformational changes associated with cofactor binding, Tetrahedron, Volume 53, Issue 35, 1 September 1997, Pages 11971-11984, ISSN 0040-4020, http://dx.doi.org/10.1016/S0040-4020(97)00710-2. (http://www.sciencedirect.com/science/article/pii/S0040402097007102)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54434
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:08 Feb 2015 03:26
Last Modified:03 Oct 2019 07:57

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