Magnuson, Jon K. and Paustian, Timothy D. and Shah, Vinod K. and Dean, Dennis R. and Roberts, Gary P. and Rees, Douglas C. and Howard, James B. (1997) Nitrogenase Iron-Molybdenum Cofactor Binding Site: Protein Conformational Changes Associated with Cofactor Binding. Tetrahedron, 53 (35). pp. 11971-11984. ISSN 0040-4020. doi:10.1016/S0040-4020(97)00710-2. https://resolver.caltech.edu/CaltechAUTHORS:20150205-140103112
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Abstract
Formation of active nitrogenase MoFe-protein requires the assembly and insertion of a unique molybdenum containing Fe:S cluster, the FeMoco. The protein acceptor is held in an open conformation by the binding of a small molecular weight protein, γ. Using selective alkylation of the protein cysteines, conformational changes associated with γ binding and cofactor insertion were evaluated and a model for the change presented. Reversible oxygen damage to the precursor is identified and evaluated in terms of the chemistry of dithionite used in the buffer as an oxygen scavenger.
| Item Type: | Article | ||||||||||
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| Contact Email Address: | phoebe@caltech.edu | ||||||||||
| Additional Information: | © 1997 Elsevier Science Ltd. Received 10 April 1997; accepted 12 June 1997. This work was supported by NSF grants MCB-9513512 (JBH), MCB-9317059 (GR), MCB-9630127 (DD) and DMB 91-18689. | ||||||||||
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| Issue or Number: | 35 | ||||||||||
| DOI: | 10.1016/S0040-4020(97)00710-2 | ||||||||||
| Record Number: | CaltechAUTHORS:20150205-140103112 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150205-140103112 | ||||||||||
| Official Citation: | Jon K. Magnuson, Timothy D. Paustian, Vinod K. Shah, Dennis R. Dean, Gary P. Roberts, Douglas C. Rees, James B. Howard, Nitrogenase iron-molybdenum cofactor binding site: Protein conformational changes associated with cofactor binding, Tetrahedron, Volume 53, Issue 35, 1 September 1997, Pages 11971-11984, ISSN 0040-4020, http://dx.doi.org/10.1016/S0040-4020(97)00710-2. (http://www.sciencedirect.com/science/article/pii/S0040402097007102) | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 54434 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | SWORD User | ||||||||||
| Deposited On: | 08 Feb 2015 03:26 | ||||||||||
| Last Modified: | 10 Nov 2021 20:33 |
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