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Crystallization and X-ray Structure Determination of Cytochrome c_2 from Rhodobacter sphaeroides in Three Crystal Forms

Axelrod, Herbert L. and Feher, George and Allen, James P. and Chirino, Arthur J. and Day, Michael W. and Hsu, Barbara T. and Rees, Douglas C. (1994) Crystallization and X-ray Structure Determination of Cytochrome c_2 from Rhodobacter sphaeroides in Three Crystal Forms. Acta Crystallographica Section D: Biological Crystallography, 50 (4). pp. 596-602. ISSN 0907-4449. https://resolver.caltech.edu/CaltechAUTHORS:20150205-141153331

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Abstract

Cytochrome c_2 serves as the secondary electron donor that reduces the photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria. Cytochrome c_2 from Rhodobacter sphaeroides has been crystallized in three different forms. At high ionic strength, crystals of a hexagonal space group (P6_122) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4_12_12) crystals were formed. The three-dimensional structures of the cytochrome in all three crystal forms have been determined by X-ray diffraction at resolutions of 2.20 Å (hexagonal), 1.95 Å, (triclinic) and 1.53 Å (tetragonal). The most significant difference observed was the binding of an imidazole molecule to the iron atom of the heme group in the hexagonal structure. This binding displaces the sulfur atom of Met 100, which forms the axial ligand in the triclinic and tetragonal structures.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1107/S0907444994001319 DOIArticle
http://scripts.iucr.org/cgi-bin/paper?S0907444994001319PublisherArticle
ORCID:
AuthorORCID
Rees, Douglas C.0000-0003-4073-1185
Contact Email Address:phoebe@caltech.edu
Additional Information:© 1994 International Union of Crystallography. Received 15 November 1993; accepted 31 January 1994.
Issue or Number:4
Record Number:CaltechAUTHORS:20150205-141153331
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150205-141153331
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54446
Collection:CaltechAUTHORS
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Deposited On:08 Feb 2015 03:02
Last Modified:03 Oct 2019 07:57

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