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The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction

Brody, Edward and Abelson, John (1985) The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. Science, 228 (4702). pp. 963-967. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20150206-114117773

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Abstract

The in vitro splicing reactions of pre-messenger RNA (pre-mRNA) in a yeast extract were analyzed by glycerol gradient centrifugation. Labeled pre-mRNA appears in a 40S peak only if the pre-mRNA undergoes the first of the two partial splicing reactions. RNA analysis after extraction of glycerol gradient fractions shows that lariat-form intermediates, molecules that occur only in mRNA splicing, are found almost exclusively in this 40S complex. Another reaction intermediate, cut 5' exon RNA, can also be found concentrated in this complex. The complex is stable even in 400 mM KCl, although at this salt concentration, it sediments at 35S and is clearly distinguishable from 40S ribosomal subunits. This complex, termed a "spliceosome," is thought to contain components necessary for mRNA splicing; its existence can explain how separated exons on pre-mRNA are brought into contact.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.3890181DOIArticle
http://www.sciencemag.org/content/228/4702/963PublisherArticle
http://www.jstor.org/stable/1694236JSTORArticle
Additional Information:© 1985 American Association for the Advancement of Science. 2 February 1985; Accepted 14 April 1985. We thank Andrew Newman, who developed the yeast in vitro mRNA splicing system, for help and encouragement during the course of this work, as well as Soo-chen Cheng, R.-J. Lin, Gloria McFarland, Marge Fidler, and Joan Steitz for advice and critical discussion. Supported by a grant from NIH.
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Issue or Number:4702
Record Number:CaltechAUTHORS:20150206-114117773
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150206-114117773
Official Citation:Brody, E., & Abelson, J. (1985). The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. Science, 228(4702), 963-967.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54483
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:06 Feb 2015 21:08
Last Modified:03 Oct 2019 07:58

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