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Synthesis of a sequence-specific DNA-cleaving peptide

Sluka, James P. and Horvath, Suzanna J. and Bruist, Michael F. and Simon, Melvin I. and Dervan, Peter B. (1987) Synthesis of a sequence-specific DNA-cleaving peptide. Science, 238 (4830). pp. 1129-1132. ISSN 0036-8075. doi:10.1126/science.3120311.

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A synthetic 52-residue peptide based on the sequence-specific DNA-binding domain of Hin recombinase (139-190) has been equipped with ethylenediaminetetraacetic acid (EDTA) at the amino terminus. In the presence of Fe(II), this synthetic EDTA-peptide cleaves DNA at Hin recombination sites. The cleavage data reveal that the amino terminus of Hin(139-190) is bound in the minor groove of DNA near the symmetry axis of Hin recombination sites. This work demonstrates the construction of a hybrid peptide combining two functional domains: sequence-specific DNA binding and DNA cleavage.

Item Type:Article
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URLURL TypeDescription DOIArticle
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1987 American Association for the Advancement of Science. 15 May 1987; accepted 31 July 1987. We thank T. A. Steitz, S. B. H. Kent, and S. Mayo for helpfuil discussions. Supported by grants from the DARPA University Research Initiative Program, the National Foundation for Cancer Research, the NSF, NIH fellowship GM-09534-02 (M.F.B.), and a National Research Service Award (T32GM07616) from the National Institute of General Medical Sciences (J.P.S.).
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
National Foundation for Cancer ResearchUNSPECIFIED
National Institute of General Medical SciencesT32GM07616
Issue or Number:4830
Record Number:CaltechAUTHORS:20150206-134713610
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Official Citation:Sluka, J., Horvath, S., Bruist, M., Simon, M., & Dervan, P. (1987). Synthesis of a sequence-specific DNA-cleaving peptide. Science, 238(4830), 1129-1132. doi: 10.1126/science.3120311
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54494
Deposited On:07 Feb 2015 00:10
Last Modified:10 Nov 2021 20:34

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