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Molecular Analysis of the Hotspot of Recombination in the Murine Major Histocompatibility Complex

Kobori, Joan A. and Strauss, Erich and Minard, Karyl and Hood, Leroy E. (1986) Molecular Analysis of the Hotspot of Recombination in the Murine Major Histocompatibility Complex. Science, 234 (4773). pp. 173-179. ISSN 0036-8075. doi:10.1126/science.3018929.

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Biological and serological assays have been used to define four subregions for the I region of the major histocompatibility complex (MHC) in the order I-A, I-B, I-J, and I-E. The I-J subregion presumably encodes the I-J polypeptide of the elusive T-cell suppressor factors. Restriction enzyme site polymorphisms and DNA sequence analyses of the I region from four recombinant mouse strains were used to localize the putative I-B and I-J subregions to a 1.0-kilobase (kb) region within the E_β gene. Sequencing this region from E_β clones derived from the two mouse strains: B10.A(3R), I-J^b and B10.A(5R), I-J^k initially used to define the I-J subregion revealed that these regions are identical, hence the distinct I-Jb and I-J^k molecules cannot be encoded by this DNA. In addition, the DNA sequence data also refute the earlier mapping of the I-B subregion. Analysis of the DNA sequences of three parental and four I region recombinants reveals that the recombinant events in three of the recombinant strains occurred within a 1-k^b region of DNA, supporting the proposition that a hotspot for recombination exists in the I region. The only striking feature of this hotspot is a tetramer repeat (AGGC)n that shows 80 percent homology to the minisatellite sequence which may facilitate recombination in human chromosomes.

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Hood, Leroy E.0000-0001-7158-3678
Additional Information:© 1986 American Association for the Advancement of Science. 2 April 1986; accepted 20 August 1986. We thank C. Graham, L. Tomich, and S. Horvath for synthesis of oligonucleotides; C. Prussin for technical assistance; D. Maloney and S. Davis for preparation of sequencing gels; M. DeBruyn for media; C. Katz and C. Elkins for typing; B. Jones for assistance in preparing figures; T. Hunkapiller, B. Arden, R. Barth, M. Kronenberg, E. Meyerowitz, H. Passmore, N. Shastri, G. Siu, L. Smith, and B. Wold for critical discussions during the course of this work and comments on the manuscript. Supported by a postdoctoral fellowship from the Arthritis Foundation (J.A.K.).
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Arthritis FoundationUNSPECIFIED
Issue or Number:4773
Record Number:CaltechAUTHORS:20150209-095005901
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Official Citation:Kobori, J., Strauss, E., Minard, K., & Hood, L. (1986). Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science, 234(4773), 173-179. doi: 10.1126/science.3018929
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ID Code:54530
Deposited On:09 Feb 2015 20:11
Last Modified:10 Nov 2021 20:34

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