CaltechAUTHORS
  A Caltech Library Service

Comparison of the X-ray Structure of Native Rubredoxin from Pyrococcus furiosus with the NMR Structure of the Zinc-Substituted Protein

Blake, Paul R. and Day, Michael W. and Hsu, Barbara T. and Joshua-Tor, Leemor and Park, Jae-Bum and Hare, Dennis R. and Adams, Michael W. W. and Rees, Douglas C. and Summers, Michael F. (1992) Comparison of the X-ray Structure of Native Rubredoxin from Pyrococcus furiosus with the NMR Structure of the Zinc-Substituted Protein. Protein Science, 1 (11). pp. 1522-1525. ISSN 0961-8368. PMCID PMC2142123. http://resolver.caltech.edu/CaltechAUTHORS:20150209-101846134

[img] PDF - Published Version
See Usage Policy.

3359Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20150209-101846134

Abstract

The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161°C, and the NMR structure of the zinc-substituted protein, determined in solution at 45°C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/pro.5560011113DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/pro.5560011113/abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142123/PubMed CentralArticle
Contact Email Address:phoebe@caltech.edu
Additional Information:© 1992 The Protein Society. Received April 29, 1992; Revised manuscript received June 19, 1992.
Subject Keywords:distance geometry; hyperthermophile; iron-sulfur protein; NMR structure; protein structure; rubredoxin; X-ray structure; zinc substitution
PubMed Central ID:PMC2142123
Record Number:CaltechAUTHORS:20150209-101846134
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150209-101846134
Official Citation:Blake, P. R., Day, M. W., Hsu, B. T., Joshua-Tor, L., Park, J.-B., Hare, D. R., Adams, M. W. W., Rees, D. C. and Summers, M. F. (1992), Comparison of the X-ray structure of native rubredoxin from pyrococcus furiosus with the NMR structure of the zinc-substituted protein. Protein Science, 1: 1522–1525. doi: 10.1002/pro.5560011113
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54535
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:10 Feb 2015 05:36
Last Modified:10 Feb 2015 05:36

Repository Staff Only: item control page