Blake, Paul R. and Day, Michael W. and Hsu, Barbara T. and Joshua-Tor, Leemor and Park, Jae-Bum and Hare, Dennis R. and Adams, Michael W. W. and Rees, Douglas C. and Summers, Michael F. (1992) Comparison of the X-ray Structure of Native Rubredoxin from Pyrococcus furiosus with the NMR Structure of the Zinc-Substituted Protein. Protein Science, 1 (11). pp. 1522-1525. ISSN 0961-8368. PMCID PMC2142123. doi:10.1002/pro.5560011113. https://resolver.caltech.edu/CaltechAUTHORS:20150209-101846134
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Abstract
The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161°C, and the NMR structure of the zinc-substituted protein, determined in solution at 45°C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.
| Item Type: | Article | ||||||||||||
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| Contact Email Address: | phoebe@caltech.edu | ||||||||||||
| Additional Information: | © 1992 The Protein Society. Received April 29, 1992; Revised manuscript received June 19, 1992. | ||||||||||||
| Subject Keywords: | distance geometry; hyperthermophile; iron-sulfur protein; NMR structure; protein structure; rubredoxin; X-ray structure; zinc substitution | ||||||||||||
| Issue or Number: | 11 | ||||||||||||
| PubMed Central ID: | PMC2142123 | ||||||||||||
| DOI: | 10.1002/pro.5560011113 | ||||||||||||
| Record Number: | CaltechAUTHORS:20150209-101846134 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150209-101846134 | ||||||||||||
| Official Citation: | Blake, P. R., Day, M. W., Hsu, B. T., Joshua-Tor, L., Park, J.-B., Hare, D. R., Adams, M. W. W., Rees, D. C. and Summers, M. F. (1992), Comparison of the X-ray structure of native rubredoxin from pyrococcus furiosus with the NMR structure of the zinc-substituted protein. Protein Science, 1: 1522–1525. doi: 10.1002/pro.5560011113 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 54535 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | SWORD User | ||||||||||||
| Deposited On: | 10 Feb 2015 05:36 | ||||||||||||
| Last Modified: | 10 Nov 2021 20:34 |
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