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Cross-linking of Nitrogenase Components: Structure and Activity of the Covalent Complex

Willing, Andreas H. and Georgiadis, Millie M. and Rees, Douglas C. and Howard, James Bryant (1989) Cross-linking of Nitrogenase Components: Structure and Activity of the Covalent Complex. Journal of Biological Chemistry, 264 (15). pp. 8499-8503. ISSN 0021-9258.

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The nitrogenase complex from Azotobacter vinelandii is composed of the MoFe protein (Av1), an α_2β_2 tetramer, and the Fe protein (Av2), a γ_2 dimer. During turnover of the enzyme, electrons are transferred from Av2 to Av1 in parallel with the hydrolysis of MgATP. Using the cross-linking reagent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, we have identified some of the properties of the complex between the two components. The cross-linking reaction was highly specific yielding a single apparent M_r = 97,000 protein. The amount of cross-linked product was essentially independent of whether MgATP or MgADP were in the reaction. Also, the amount was maximum at high ratios of Av2 to Av1. The M_r = 97,000 protein was characterized by amino acid analysis and Edman degradation and was found to be consistent with a 1:1 complex of an Av2 γ subunit and an Av1 β subunit (the amino terminal serine subunit). The complex was no longer active in the nitrogenase reaction which supports, but does not prove, the requirement for dissociation of the complex after each electron transferred. Nitrogenase activity and cross-linking were inhibited in an identical way by NaCl, which suggests that electrostatic forces are critical to the formation of the electron transfer complex.

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Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 1989 American Society for Biochemistry and Molecular Biology. Received for publication, December 23, 1988. This work was supported by Grants GM 34321 (to J.B. H.) and GM 31299 (to D. C.R.) from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement", in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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NIHGM 34321
NIHGM 31299
Issue or Number:15
Record Number:CaltechAUTHORS:20150209-102138428
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Official Citation:A H Willing, M M Georgiadis, D C Rees, and J B Howard Cross-linking of nitrogenase components. Structure and activity of the covalent complex. J. Biol. Chem. 1989 264: 8499-8503.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54537
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Deposited On:10 Feb 2015 05:31
Last Modified:03 Oct 2019 07:58

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