CaltechAUTHORS
  A Caltech Library Service

Echinomycin Binding Sites on DNA

Van Dyke, Michael M. and Dervan, Peter B. (1984) Echinomycin Binding Sites on DNA. Science, 225 (4667). pp. 1122-1127. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20150209-103133277

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150209-103133277

Abstract

The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A ^(32)P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA. Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.6089341DOIArticle
http://www.sciencemag.org/content/225/4667/1122.abstractPublisherArticle
http://www.jstor.org/stable/1693724JSTORArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1984 American Association for the Advancement of Science. 8 May 1984; accepted 28 June 1984. Supported by the American Cancer Society research grant number NP-428, an unrestricted grant from Burroughs Wellcome Co. (P.B.D.), and a National Research Service Award (GM-07616) (to M.W.V.). We thank Dr. A. Rich for informing us of his results before publication. Address correspondence to P .B.D.
Funders:
Funding AgencyGrant Number
American Cancer SocietyNP-428
Burroughs WellcomeUNSPECIFIED
NIHGM07616
Issue or Number:4667
Record Number:CaltechAUTHORS:20150209-103133277
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150209-103133277
Official Citation:Echinomycin binding sites on DNA MM Van Dyke and PB Dervan Science 14 September 1984: 225 (4667), 1122-1127. [DOI:10.1126/science.6089341]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54542
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Feb 2015 20:21
Last Modified:26 Nov 2019 11:15

Repository Staff Only: item control page