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Echinomycin Binding Sites on DNA

Van Dyke, Michael M. and Dervan, Peter B. (1984) Echinomycin Binding Sites on DNA. Science, 225 (4667). pp. 1122-1127. ISSN 0036-8075. doi:10.1126/science.6089341.

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The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A ^(32)P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA. Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).

Item Type:Article
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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1984 American Association for the Advancement of Science. 8 May 1984; accepted 28 June 1984. Supported by the American Cancer Society research grant number NP-428, an unrestricted grant from Burroughs Wellcome Co. (P.B.D.), and a National Research Service Award (GM-07616) (to M.W.V.). We thank Dr. A. Rich for informing us of his results before publication. Address correspondence to P .B.D.
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American Cancer SocietyNP-428
Burroughs WellcomeUNSPECIFIED
Issue or Number:4667
Record Number:CaltechAUTHORS:20150209-103133277
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Official Citation:Echinomycin binding sites on DNA MM Van Dyke and PB Dervan Science 14 September 1984: 225 (4667), 1122-1127. [DOI:10.1126/science.6089341]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54542
Deposited By: Ruth Sustaita
Deposited On:09 Feb 2015 20:21
Last Modified:10 Nov 2021 20:35

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