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Structure of the Reaction Center from Rhodopseudomonas sphaeroides R-26 and 2.4.1: Symmetry Relations and Sequence Comparisons between Different Species

Komiya, H. and Yeates, T. O. and Rees, D. C. and Allen, J. P. and Feher, G. (1988) Structure of the Reaction Center from Rhodopseudomonas sphaeroides R-26 and 2.4.1: Symmetry Relations and Sequence Comparisons between Different Species. Proceedings of the National Academy of Sciences of the United States of America, 85 (23). pp. 9012-9016. ISSN 0027-8424. PMCID PMC282652. http://resolver.caltech.edu/CaltechAUTHORS:20150209-103422026

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Abstract

Photosynthetic reaction centers from purple bacteria exhibit an approximate twofold symmetry axis, which relates both the cofactors and the L and M subunits. For the reaction center from Rhodobacter sphaeroides, deviations from this twofold symmetry axis have been quantitated by superposing, by a 180 degrees rotation, the cofactors of the B branch onto the A branch and the M subunit onto the L subunit. An alignment of the sequences of the L and M subunits from four purple bacteria, one green bacterium, and the D_1 and D_2 subunits of a photosystem II-containing green alga is presented. The residues that are conserved in all six species are shown in relation to the structure of Rb. sphaeroides and their possible role in the function of the reaction center is discussed. A method is presented for characterizing the exposure of α-helices to the membrane based on the periodicity of conserved residues. This method may prove useful for modeling the three-dimensional structures of membrane proteins.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/85/23/9012.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC282652/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0002-1076-1503
Additional Information:© 1988 National Academy of Sciences. Contributed by G. Feher, August 22, 1988. We thank E. Abresch for the preparation of the RCs and M. Y. Okamura, L. DeAntonio, and A. Chirino, for helpful discussions. This work was supported by grants from the National Institutes of Health (AM36053, GM13191, GM31875) and the National Science Foundation (DMB85-18922 and a Presidential Young Investigators Award). D.C.R. is an A. P. Sloan Research Fellow. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U .S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAM36053
NIHGM13191
NIHGM31875
NSFDMB85-18922
NSF Presidential Young Investigator AwardUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:bacterial photosynthesis; membrane protein structure; x-ray diffraction
PubMed Central ID:PMC282652
Record Number:CaltechAUTHORS:20150209-103422026
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150209-103422026
Official Citation:H Komiya, T O Yeates, D C Rees, J P Allen, and G Feher Structure of the reaction center from Rhodobacter sphaeroides R-26 and 2.4.1: symmetry relations and sequence comparisons between different species PNAS 1988 85 (23) 9012-9016
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54543
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:10 Feb 2015 05:26
Last Modified:22 Nov 2017 00:36

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