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Structure of the Reaction Center from Rhodopseudomonas sphaeroides R-26: Protein-Cofactor (Quinones and Fe^(2+)) Interactions

Allen, J. P. and Feher, G. and Yeates, T. O. and Komiya, H. and Rees, D. C. (1988) Structure of the Reaction Center from Rhodopseudomonas sphaeroides R-26: Protein-Cofactor (Quinones and Fe^(2+)) Interactions. Proceedings of the National Academy of Sciences of the United States of America, 85 (22). pp. 8487-8491. ISSN 0027-8424. PMCID PMC282483. http://resolver.caltech.edu/CaltechAUTHORS:20150209-103511705

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Abstract

The three-dimensional structure of the reaction center (RC) from Rhodobacter sphaeroides has been determined by x-ray diffraction to a resolution of 2.8 Å with an R value of 24%. The interactions of the protein with the primary quinone, Q_A, secondary quinone, Q_B, and the nonheme iron are described and compared to those of RCs from Rhodopseudomonas viridis. Structural differences between the Q_A and Q_B environments that contribute to the function of the quinones (the electron transfer from Q_A- to Q_B and the charge recombination of Q_A-, Q_B- with the primary donor) are delineated. The protein residues that may be involved in the protonation of Q_B are identified. A pathway for the doubly reduced Q_B to dissociate from the RC is proposed. The interactions between QB and the residues that have been changed in herbicide-resistant mutants are described. The environment of the nonheme iron is compared to the environments of metal ions in other proteins.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/85/22/8487.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC282483/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0002-1076-1503
Additional Information:© 1988 National Academy of Sciences. Contributed by G. Feher, August 5, 1988. We thank E. Abresch for preparation of the RCs and M. Y. Okamura, W. Lubitz, and A. Chirino for helpful discussions. This work was supported by grants from the National Institutes of Health (AM36053, GM13191, GM31875) and the National Science Foundation (DMB85-18922 and a Presidential Young Investigators Award). D.C.R. is an A. P. Sloan research fellow. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAM36053
NIHGM13191
NIHGM31875
NSFDMB85-18922
NSF Presidential Young Investigator AwardUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:bacterial photosynthesis; membrane protein structure; x-ray diffraction
PubMed Central ID:PMC282483
Record Number:CaltechAUTHORS:20150209-103511705
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150209-103511705
Official Citation:J P Allen, G Feher, T O Yeates, H Komiya, and D C Rees Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactions PNAS 1988 85 (22) 8487-8491
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54544
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:10 Feb 2015 05:25
Last Modified:22 Nov 2017 00:37

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