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Structure of the reaction center from Rhodobacter sphaeroides R-26: membrane-protein interactions

Yeates, T. O. and Komiya, H. and Rees, D. C. and Allen, J. P. and Feher, G. (1987) Structure of the reaction center from Rhodobacter sphaeroides R-26: membrane-protein interactions. Proceedings of the National Academy of Sciences of the United States of America, 84 (18). pp. 6438-6442. ISSN 0027-8424. PMCID PMC299092. https://resolver.caltech.edu/CaltechAUTHORS:20150209-103902990

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Abstract

The energetics of membrane-protein interactions are analyzed with the three-dimensional model of the photosynthetic reaction center (RC) from Rhodobacter sphaeroides. The position of the RC in the membrane and the thickness of the membrane were obtained by minimizing the hydrophobic energy with the energy function of Eisenberg and McLachlan. The 2-fold symmetry axis that relates the L and M subunits is, within the accuracy of 5 degrees, parallel to the normal of the membrane. The thickness of the membrane is estimated to be 40-45 Å. Residues that are exposed to the membrane are relatively poorly conserved in the sequences of homologous RC proteins. The surface area of the RC is comparable to the surface areas of water-soluble proteins of similar molecular weight. The volumes of interior atoms in the RC are also similar to those of water-soluble proteins, indicating the same compact packing for both types of proteins. The electrostatic potential of the cofactors was calculated. The results show an asymmetry in the potential between the two possible pathways of electron transfer, with the A branch being preferred electrostatically.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/84/18/6438.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC299092/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0003-4073-1185
Additional Information:© 1987 National Academy of Sciences. Contributed by George Feher, June 2, 1987. We thank David Eisenberg for helpful discussions. This work was supported by grants from the National Institutes of Health (AM36053, GM13191, GM31875), the National Science Foundation (DMB85-18922, and a Presidential Young Investigators Award), and the Chicago Community Trust/ Searle Scholars Program. D.C.R. is an A. P. Sloan research fellow. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAM36053
NIHGM13191
NIHGM31875
NSFDMB85-18922
NSF Presidential Young Investigator AwardUNSPECIFIED
Chicago Community TrustUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:bacterial photosynthesis; membrane protein structure; membrane energetics
Issue or Number:18
PubMed Central ID:PMC299092
Record Number:CaltechAUTHORS:20150209-103902990
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150209-103902990
Official Citation:T O Yeates, H Komiya, D C Rees, J P Allen, and G Feher Structure of the reaction center from Rhodobacter sphaeroides R-26: membrane-protein interactions PNAS 1987 84 (18) 6438-6442
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54547
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:10 Feb 2015 05:21
Last Modified:03 Oct 2019 07:58

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