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Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits

Allen, J. P. and Feher, G. and Yeates, T. O. and Komiya, H. and Rees, D. C. (1987) Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits. Proceedings of the National Academy of Sciences of the United States of America, 84 (17). pp. 6162-6166. ISSN 0027-8424. PMCID PMC299029. https://resolver.caltech.edu/CaltechAUTHORS:20150209-104007322

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Abstract

The three-dimensional structure of the protein subunits of the reaction center (RC) of Rhodobacter sphaeroides has been determined by x-ray diffraction at a resolution of 2.8 Å with an R factor of 26%. The L and M subunits each contain five transmembrane helices and several helices that do not span the membrane. The L and M subunits are related to each other by a 2-fold rotational symmetry axis that is approximately the same as that determined for the cofactors. The H subunit has one transmembrane helix and a globular domain on the cytoplasmic side, which contains a helix that does not span the membrane and several β-sheets. The structural homology with RCs from other purple bacteria is discussed. A structure of the complex formed between the water soluble cytochrome c_2 and the RC from Rb. sphaeroides is proposed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/84/17/6162PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC299029/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0003-4073-1185
Additional Information:© 1987 National Academy of Sciences. Contributed by G. Feher. May 18. 1987. We thank E. Abresch for the preparation of the RCs. This work was supported by grants from the National Institutes of Health (AM36053, GM13191, GM31299, and GM07185), the National Science Foundation (DMB85-18922J, a Presidential Young Investigators Award, and the Chicago Community Trust/Searle Scholars Program. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAM36053
NIHGM13191
NIHGM31299
NIHGM07185
NSFDMB85-18922
Chicago Community TrustUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Subject Keywords:bacterial photosynthesis; membrane protein structure; x-ray diffraction; cytochrome c-reaction center complex
Issue or Number:17
PubMed Central ID:PMC299029
Record Number:CaltechAUTHORS:20150209-104007322
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150209-104007322
Official Citation:J P Allen, G Feher, T O Yeates, H Komiya, and D C Rees Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunits PNAS 1987 84 (17) 6162-6166
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54548
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:09 Feb 2015 22:37
Last Modified:03 Oct 2019 07:58

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