CaltechAUTHORS
  A Caltech Library Service

The refinement of the haemagglutinin membrane glycoprotein of influenza virus

Knossow, M. and Lewis, M. and Rees, D. and Wilson, I. A. and Skehel, J. J. and Wiley, D. C. (1986) The refinement of the haemagglutinin membrane glycoprotein of influenza virus. Acta crystallographica. Section B, Structural science, 42 (6). pp. 627-632. ISSN 0108-7681. https://resolver.caltech.edu/CaltechAUTHORS:20150209-104311982

[img] PDF - Published Version
See Usage Policy.

548Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150209-104311982

Abstract

The crystal structure of the haemagglutinin glycoprotein, a trimer from the membrane of influenza virus, has been refined at 3.0 Å resolution to an R factor of 20.4%. The first 23 cycles were carried out on coordinates of an averaged monomer determined from a non-crystallographically threefold-symmetry-averaged electron density map. The contribution of structure factors to the least-squares equations were determined from non-crystallographically averaged gradient difference and curvature Fourier maps. The refinement was restrained using the Hendrickson & Konnert algorithm. These initial cycles were followed by 25 cycles of refinement with trigonometric evaluation of the derivatives on the complete trimer (208 422 daltons) on a Cray 1/S. Forty-eight water molecules and portions of five N-linked oligosaccharides were identified.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1107/S0108768186097628 DOIArticle
http://scripts.iucr.org/cgi-bin/paper?S0108768186097628PublisherArticle
ORCID:
AuthorORCID
Rees, D.0000-0003-4073-1185
Additional Information:© 1986 International Union of Crystallography. Received 17 January 1986; accepted 30 June 1986. MK acknowledges support from the MRT (IPB/78), the CNRS (ATP France-USA 3/84), the Centre de Calcul Vectoriel pour le Recherche for Cray 1/S time, and the use of the LURE graphics facility. DCW acknowledges support from NIH grant No. AI 13654.
Funders:
Funding AgencyGrant Number
NIHAI 13654
MRTIPB/78
Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20150209-104311982
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150209-104311982
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54552
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:09 Feb 2015 21:52
Last Modified:03 Oct 2019 07:58

Repository Staff Only: item control page