Knossow, M. and Lewis, M. and Rees, D. and Wilson, I. A. and Skehel, J. J. and Wiley, D. C. (1986) The refinement of the haemagglutinin membrane glycoprotein of influenza virus. Acta crystallographica. Section B, Structural science, 42 (6). pp. 627-632. ISSN 0108-7681. doi:10.1107/S0108768186097628. https://resolver.caltech.edu/CaltechAUTHORS:20150209-104311982
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Abstract
The crystal structure of the haemagglutinin glycoprotein, a trimer from the membrane of influenza virus, has been refined at 3.0 Å resolution to an R factor of 20.4%. The first 23 cycles were carried out on coordinates of an averaged monomer determined from a non-crystallographically threefold-symmetry-averaged electron density map. The contribution of structure factors to the least-squares equations were determined from non-crystallographically averaged gradient difference and curvature Fourier maps. The refinement was restrained using the Hendrickson & Konnert algorithm. These initial cycles were followed by 25 cycles of refinement with trigonometric evaluation of the derivatives on the complete trimer (208 422 daltons) on a Cray 1/S. Forty-eight water molecules and portions of five N-linked oligosaccharides were identified.
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| Additional Information: | © 1986 International Union of Crystallography. Received 17 January 1986; accepted 30 June 1986. MK acknowledges support from the MRT (IPB/78), the CNRS (ATP France-USA 3/84), the Centre de Calcul Vectoriel pour le Recherche for Cray 1/S time, and the use of the LURE graphics facility. DCW acknowledges support from NIH grant No. AI 13654. | |||||||||
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| Issue or Number: | 6 | |||||||||
| DOI: | 10.1107/S0108768186097628 | |||||||||
| Record Number: | CaltechAUTHORS:20150209-104311982 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150209-104311982 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 54552 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | SWORD User | |||||||||
| Deposited On: | 09 Feb 2015 21:52 | |||||||||
| Last Modified: | 10 Nov 2021 20:35 |
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