Clark-Lewis, Ian and Aebersold, Ruedi and Ziltener, Herman and Schrader, John W. and Hood, Leroy E. and Kent, Stephen B. H. (1986) Automated Chemical Synthesis of a Protein Growth Factor for Hemopoietic Cells, Interleukin-3. Science, 231 (4734). pp. 134-139. ISSN 0036-8075. doi:10.1126/science.3079915. https://resolver.caltech.edu/CaltechAUTHORS:20150209-113416478
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Abstract
Interleukin-3 (IL-3), a protein of 140 amino acids, was chemically synthesized by means of an automated peptide synthesizer and was shown to have the biological activities attributed to native IL-3. Assays of synthetic analogues established that an amino terminal fragment has detectable IL-3 activity, but that the stable tertiary structure of the complete molecule was required for full activity. The results demonstrate that automated peptide synthesis can be applied to the study of the structure and function of proteins.
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| Additional Information: | © 1986 American Association for the Advancement of Science. 7 October 1985; accepted 2 December 1985. Supported by research grants from the National Institutes of Health, the Monsanto Company, and Upjohn Pharmaceuticals. Additional support was obtained from the National Health and Medical Research Council, Canberra, Australia; NIH grant R01-CA38684-0l (J.W.S.); a C.]. Martin Traveling Fellowship from the National Health and Medical Research Council, Canberra, Australia (I.C.L.), an EMBO long-term fellowship (R.A.) and fellowships from the Swiss National Foundation (R.A. and H.Z.). We thank C. Davis and J. Kim for expert technical assistance. | ||||||||||||||||
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| Issue or Number: | 4734 | ||||||||||||||||
| DOI: | 10.1126/science.3079915 | ||||||||||||||||
| Record Number: | CaltechAUTHORS:20150209-113416478 | ||||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150209-113416478 | ||||||||||||||||
| Official Citation: | Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3 I Clark-Lewis, R Aebersold, H Ziltener, JW Schrader, LE Hood, and SB Kent Science 10 January 1986: 231 (4734), 134-139. [DOI:10.1126/science.3079915] | ||||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||||
| ID Code: | 54556 | ||||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||||
| Deposited By: | Ruth Sustaita | ||||||||||||||||
| Deposited On: | 09 Feb 2015 20:10 | ||||||||||||||||
| Last Modified: | 10 Nov 2021 20:35 |
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