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Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen

Milich, David R. and Thornton, George B. and Neurath, A. Robert and Kent, Steven B. and Michel, Marie-Louise and Tiollais, Pierre and Chisari, Francis V. (1985) Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen. Science, 228 (4704). pp. 1195-1199. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150209-121616968

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Abstract

The 55 codons upstream of the gene sequence encoding the hepatitis B surface antigen (HBsAg) are called the pre-S(2) region. It has been proposed that polypeptides of high molecular weight that contain the pre-S(2) region should be included in future hepatitis B virus (HBV) vaccines. The pre-S(2) region and the S gene product [25 kilodalton (kD)] together compose a polypeptide of high molecular weight (33 kD). As an initial attempt to determine the relevance of the 33-kD polypeptide to development of an HBV vaccine, the murine immune response to pre-S(2)-encoded determinants as compared to S-encoded determinants on the same polypeptide was examined. The results indicate (i) the pre-S(2) region is significantly more immunogenic than the S region of HBsAg, (ii) the 26 amino acid residues at the NH2-terminus of the 33-kD polypeptide represent a dominant antibody binding site on the pre-S(2) region, (iii) the immune response to the pre-S(2) region is regulated by H-2-linked genes distinct from those that regulate the response to the S region, and (iv) immunization of an S region nonresponder strain with HBV envelope particles that contain both the pre-S(2) and S regions can circumvent nonresponsiveness to the S region.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.2408336DOIArticle
http://www.sciencemag.org/content/228/4704/1195PublisherArticle
http://www.jstor.org/stable/1694930JSTORArticle
Additional Information:© 1985 American Association for the Advancement of Science. Publication No. 3795-BCR from the Scripps Clinic and Research Foundation. 1 February 1985; 17 April 1985. We thank M. McNamara and A. McLachlan for discussion of the manuscript, R. Louie and Cutter Laboratories for the HBsAG/p25 preparation, J. Hughes for technical assistance, and J. Verenini for preparation of the manuscript. This is publication No. 3795-BCR from the Scripps Clinic and Research Foundation, supported by NIH grants AI 20720, AI 00585, and AI 20001.
Funders:
Funding AgencyGrant Number
NIHAI 20720
NIHAI 00585
NIHAI 20001
Record Number:CaltechAUTHORS:20150209-121616968
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150209-121616968
Official Citation:Milich, D., Thornton, G., Neurath, A., Kent, S., Michel, M., Tiollais, P., & Chisari, F. (1985). Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen. Science, 228(4704), 1195-1199. doi: 10.1126/science.2408336
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54559
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:09 Feb 2015 21:22
Last Modified:09 Feb 2015 21:22

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