Molecular Evolution Activities
 

This is a comprehensive bibliography (under construction) of primary and secondary sources on the neutral theory of molecular evolution. It currently covers the period 1973-2001.

Author :

Wang, I. N.;Dykhuizen, D. E.

Year :

2001

Title :

Variation of enzyme activities at a branched pathway involved in the utilization of gluconate in Escherichia coli

Journal :

Evolution

Volume :

55

Issue :

5

Pages :

897-908

Date :

May

Short Title :

Variation of enzyme activities at a branched pathway involved in the utilization of gluconate in Esc

Alternate Journal :

Evolution

Custom 2 :

ISI:000169403100006

Abstract :

Twenty-four strains of Escherichia coli from the ECOR collection were characterized for growth rate in gluconate minimal salts medium and for V-max and K-m of the three enzymes (gluconokinase, 6-phosphogluconate dehydrogenase, and 6- phosphogluconate dehydratase) that form a branch point for the utilization of gluconate. A total of 11 characters-growth rate, three V-max values, four K-m values, and three V-max/K-m values-were determined for these 24 ECOR strains. Most of the characters were normally distributed. Statistical tests showed that growth rate is significantly less variable than enzyme activities. Also, analyses of variance showed significant differences among strains and among the extant five genetic groups of E. coli for the characters measured. A Mantel test showed that, for some characters, closely related strains shared similar character values. Two hypotheses regarding the relationships between growth rate and enzyme activity and between various enzyme activities were tested. None of the expected correlations between growth rate and enzyme activity or between enzyme activities was detected. The results were discussed in terms of metabolic control analysis and neutral theory.

Notes :

Times Cited: 0 444MK EVOLUTION
 -- contributed by John Beatty, March 29, 2002