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Structural Homology of Reaction Centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as Determined by X-ray Diffraction

Allen, J. P. and Feher, G. and Yeates, T. O. and Rees, D. C. and Deisenhofer, J. and Michel, H. and Huber, R. (1986) Structural Homology of Reaction Centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as Determined by X-ray Diffraction. Proceedings of the National Academy of Sciences of the United States of America, 83 (22). pp. 8589-8593. ISSN 0027-8424. PMCID PMC386976. http://resolver.caltech.edu/CaltechAUTHORS:20150210-094402318

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Abstract

Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P2_12_12_1, have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/83/22/8589PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC386976/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0002-1076-1503
Additional Information:© 1986 National Academy of Sciences. Contributed by G. Feher, July 24, 1986. We thank E. Abresch for the preparation of the RCs and H. Komiya for assistance with the data analysis. This work was supported by grants from the National Institutes of Health (AM36053, GM13191, and GM31875) and the Chicago Community Trust/Searle Scholars Program. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAM36053
NIHGM13191
NIHGM31875
Chicago Community TrustUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Subject Keywords:bacterial photosynthesis; Patterson search method; membrane protein structure; structure of bacterial reaction centers
PubMed Central ID:PMC386976
Record Number:CaltechAUTHORS:20150210-094402318
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150210-094402318
Official Citation:J P Allen, G Feher, T O Yeates, D C Rees, J Deisenhofer, H Michel, and R Huber Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction PNAS 1986 83 (22) 8589-8593
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54636
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:11 Feb 2015 01:02
Last Modified:22 Nov 2017 00:26

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