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Complex Formation between Flavodoxin and Cytochrome c: Cross-linking Studies

Dickerson, Joyce L. and Kornuc, John J. and Rees, Douglas C. (1985) Complex Formation between Flavodoxin and Cytochrome c: Cross-linking Studies. Journal of Biological Chemistry, 260 (8). pp. 5175-5178. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:20150210-094659168

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Abstract

Complex formation between Azotobacter vinelandii flavodoxin and horse cytochrome c has been demonstrated through cross-linking studies with dimethyl suberimidate, dimethyl adipimidate, 1-ethyl-3-(3-di-methylaminopropyl)carbodiimide, and dimethyl-3,3'-dithiobispropionimidate. Essentially quantitative cross-linking of cytochrome c and flavodoxin was observed at low ionic strengths with the carbodiimide cross-linking reagent. An association constant of 4 X 10^4 M^(-1) was obtained between cytochrome c and flavodoxin at 88 mM ionic strength from analysis of the cross-linking studies. This value is similar to the association constant determined kinetically during the electron transfer reaction between cytochrome c and flavodoxin (Simondsen, R.P., Weber, P.C., Salemme, F.R., and Tollin, G. (1982) Biochemistry 21, 6366-6375), and suggests that the cross-linked complex may be similar to the precursor complex identified kinetically. A structural model for the flavodoxin-cytochrome c complex proposed by these workers is shown to be compatible with the present cross-linking results.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jbc.org/content/260/8/5175.abstractPublisherArticle
ORCID:
AuthorORCID
Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 1985 American Society for Biochemistry and Molecular Biology. Received for publication, March 26, 1984. We would like to thank Nguyet Tran for her excellent technical assistance, and Drs. S. G. Clarke, D.S. Eisenberg, and A. Fowler for helpful discussions and instrumentation. This work was supported by National Institutes of Health Grant GM31875, a Dreyfus Foundation Starter Grant in Chemistry, and the Chicago Community Trust/Searle Scholars Program. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHGM31875
Camille and Henry Dreyfus FoundationUNSPECIFIED
Chicago Community TrustUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Issue or Number:8
Record Number:CaltechAUTHORS:20150210-094659168
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150210-094659168
Official Citation:J L Dickerson, J J Kornuc, and D C Rees Complex formation between flavodoxin and cytochrome c. Cross-linking studies. J. Biol. Chem. 1985 260: 5175-8
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:54639
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:10 Feb 2015 18:08
Last Modified:03 Oct 2019 07:59

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