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Linear Structure of the Oligosaccharide Chains in α_1-Protease Inhibitor Isolated from Human Plasma

Chan, Shung Kai and Rees, Douglas C. and Li, Su-Chen and Li, Yu-Teh (1976) Linear Structure of the Oligosaccharide Chains in α_1-Protease Inhibitor Isolated from Human Plasma. Journal of Biological Chemistry, 251 (2). pp. 471-476. ISSN 0021-9258.

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Two glycopeptides present in equal amounts were isolated from a pronase digest of alpha1-protease inhibitor of human plasma by gel filtration on Sephadex G-50 and chromatography on DEAE-cellulose. The carbohydrate side chains in both glycopeptides are linked through asparaginyl residues. The glycopeptides were digested sequentially with specific glycosidases; and after each step, the released sugars as well as the composition of the residual peptides were determined. The linear structures of these glycopeptides deduced from these data are shown below. Based on the total carbohydrate content of the intact protein and with these structural data, it is postulated that 4 oligosaccharide units are attached to 1 molecule of the protein; 2 of these were represented as in Equation 1, the other 2 as in Equation 2.

Item Type:Article
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Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 1976 American Society for Biochemistry and Molecular Biology. Received for publication, December 9, 1974 and in revised form, May 1, 1975. This work was supported in part by Research Grants HL 16166 from the National Institutes of Health (to S. K. C.) and National Science Foundation Grant GB 43571 (to Y.T. L.).
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NIHHL 16166
NSFGB 43571
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Record Number:CaltechAUTHORS:20150210-100848506
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ID Code:54660
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Deposited On:10 Feb 2015 20:27
Last Modified:03 Oct 2019 07:59

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