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Analysis of SCG10 Gene Expression in Transgenic Mice Reveals That Neural Specificity Is Achieved through Selective Derepression

Wuenschell, Carol W. and Mori, Nozomu and Anderson, David J. (1990) Analysis of SCG10 Gene Expression in Transgenic Mice Reveals That Neural Specificity Is Achieved through Selective Derepression. Neuron, 4 (4). pp. 595-602. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20150224-110701073

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Abstract

SCG10 is a neural-specific, growth-associated protein that is broadly expressed in the embryonic central and peripheral nervous systems. Transgenic mice harboring a chimeric gene containing 4 kb of SCG10 5′ flanking DNA fused to the bacterial CAT gene exhibit expression in brain but not in nonneuronal tissues. A low level of expression is detected in adrenal gland as well, consistent with the behavior of endogenous SCG10. Such a transgene is also activated at the same relative stage of embryonic development as its endogenous counterpart. Deletion of the 5′-most 3.7 kb of SCG10 sequence yields deregulated expression of the transgene in numerous nonneuronal tissues, although expression remains highest in brain. In contrast to other tissue-specific genes, therefore, the specificity of SCG10 expression appears to be achieved predominantly through selective repression in nonneuronal tissues.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0896-6273(90)90117-XDOIArticle
http://www.sciencedirect.com/science/article/pii/089662739090117XPublisherArticle
Additional Information:© 1990 by Cell Press. Received 26 December 1989, Revised 2 February 1990, Available online 19 April 2004. We are grateful to Jesse Dausman for instruction in the transgenic mouse technique, to Jane Johnson for helpful suggestions, and to Carol Readhead for managing the Transgenic Animal Facility at Caltech. We thank Steven Padilla for technical assistance and Barbara Wold and Chris Schoenherr for constructive comments on the manuscript. This work was supported by a Markey Internal Grant in Developmental Biology and by Searle Scholars and National Science Foundation Presidential Young Investigator awards to D. J. A. C. W.W. was supported by a National Institutes of Health postdoctoral fellowship. D. j. A. is an Assistant Investigator, and C. W.W. and N. M. are Associates, of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
Lucille P. Markey Charitable TrustUNSPECIFIED
Searle ScholarsUNSPECIFIED
NSF Presidential Young Investigator AwardUNSPECIFIED
NIH postdoctoral fellowshipUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20150224-110701073
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150224-110701073
Official Citation:Carol W. Wuenschell, Nozomu Mori, David J. Anderson, Analysis of SCG10 gene expression in transgenic mice reveals that neural specificity is achieved through selective derepression, Neuron, Volume 4, Issue 4, April 1990, Pages 595-602, ISSN 0896-6273, http://dx.doi.org/10.1016/0896-6273(90)90117-X. (http://www.sciencedirect.com/science/article/pii/089662739090117X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55147
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:24 Feb 2015 19:38
Last Modified:03 Oct 2019 08:03

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