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The cellular function of MASH1 in autonomic neurogenesis

Sommer, Lukas and Shah, Nirao and Rao, Mahendra and Anderson, David J. (1995) The cellular function of MASH1 in autonomic neurogenesis. Neuron, 15 (6). pp. 1245-1258. ISSN 0896-6273. doi:10.1016/0896-6273(95)90005-5.

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Using primary cultures and immortalized multipotential stem cell lines derived from wild-type and Mash1 mutant neural crest cells, we have analyzed the cellular function of MASH1 in autonomic neurogenesis. We present evidence for the existence of a precursor expressing MASH1 and neuronal markers such as neurofilament, neuron-specific tubulin, and tetanus toxin receptor. This cell has a non neuronal morphology. Differentiation of this precursor to neurons that express markers such as SCG10, peripherin, and neuron-specific enolase is dependent upon MASH1 function. These data imply that the differentiation of autonomic neurons from uncommitted neural crest cells occurs in several sequential steps. Moreover, they suggest that MASH1 does not commit multipotent cells to a neural fate, like its Drosophila achaefe-scute counter-parts, but rather promotes the differentiation of a committed neuronal precursor.

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Anderson, David J.0000-0001-6175-3872
Additional Information:© 1995 by Cell Press. Under an Elsevier user license. Received 18 June 1995, Revised 20 October 1995, Available online 22 October 2003. We thank Liching Lo for providing anti·c·RET antibodies and helpful technical comments, Tony Frankfurter for TuJ1 antibody, Monica M. Oblinger for peripherin and NF160 probes, Jean-Pierre Julien for the NF68 probe, Andy Groves for advice with in situ hybridization, and Alice Paquette for kindly providing the dispase/collagenase protocol for the isolation of mouse neural tubes. We are grateful to Rochelle Diamond and the Caltech Flow Cytometry facility for helpful advice and assistance with cell sorting procedures and to Kai Zinn and Richard Axel for critical comments on the manuscript. L. S. was supported by the Swiss Foundation for Medical and Biological Scholarships. M. S. R. was an Associate and D. J. A. is an Associate Investigator of the Howard Hughes Medical Institute. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.
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Swiss Foundation for Medical and Biological ScholarshipsUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20150225-073128058
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Official Citation:Lukas Sommer, Nirao Shah, Mahendra Rao, David J. Anderson, The cellular function of MASH1 in autonomic neurogenesis, Neuron, Volume 15, Issue 6, December 1995, Pages 1245-1258, ISSN 0896-6273, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55174
Deposited By: Ruth Sustaita
Deposited On:25 Feb 2015 15:59
Last Modified:10 Nov 2021 20:42

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