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Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation

Anderson, Dawn J. and Morris, Christina J. and Nunn, David N. and Anthony, Christopher and Lidstrom, Mary E. (1990) Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation. Gene, 90 (1). pp. 173-176. ISSN 0378-1119. https://resolver.caltech.edu/CaltechAUTHORS:20150225-084708010

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Abstract

The nucleotide sequence has been determined for two genes involved in methanol oxidation in the facultative methylotroph, Methylobacterium extorquens AM1. The two genes are moxF, encoding the 66-kDa subunit of the methanol dehydrogenase and moxJ, located immediately downstream from moxF, which encodes a 30-kDa protein with unknown function. This information completes the sequence of the 5.86-kb XhoI-SalI fragment containing the moxFJGI region in M. extorquens AM1, and the structure of this gene cluster is presented. Evidence is presented that moxJ is also present in Paracoccus denitrificans. The aa sequence of MoxJ has provided little information concerning its function, but it does appear to contain a signal sequence suggesting a periplasmic location.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0378-1119(90)90457-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0378111990904573PublisherArticle
Additional Information:© 1990 Elsevier Science Publishers B.V. Received 5 December 1989, Revised 17 February 1990, Accepted 20 February 1990, Available online 19 December 2002. Received by M. Bagdasarian. This work was supported by a grant to M.E.L. from the US Department of Energy (No. DE-FG03-87ER13753). D.N.N. was supported by an NIH Post-doctoral fellowship. C.A. was supported by SERC.
Funders:
Funding AgencyGrant Number
Department of Energy (DOE)DE-FG03-87ER13753
NIH Postdoctoral FellowshipUNSPECIFIED
Science and Engineering Research Council (UK)UNSPECIFIED
Subject Keywords:Methanol dehydrogenase; methylotroph; signal peptide; codon usage; recombinant DNA
Issue or Number:1
Record Number:CaltechAUTHORS:20150225-084708010
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150225-084708010
Official Citation:Dawn J. Anderson, Christina J. Morris, David N. Nunn, Christopher Anthony, Mary E. Lidstrom, Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation, Gene, Volume 90, Issue 1, 31 May 1990, Pages 173-176, ISSN 0378-1119, http://dx.doi.org/10.1016/0378-1119(90)90457-3. (http://www.sciencedirect.com/science/article/pii/0378111990904573)
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ID Code:55186
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:25 Feb 2015 17:16
Last Modified:03 Oct 2019 08:03

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