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Expression of EphrinB2 Identifies a Stable Genetic Difference Between Arterial and Venous Vascular Smooth Muscle as Well as Endothelial Cells, and Marks Subsets of Microvessels at Sites of Adult Neovascularization

Shin, Donghun and Garcia-Cardena, Guillermo and Hayashi, Shin-Ichiro and Gerety, Sebastian and Asahara, Takayuki and Stavrakis, George and Isner, Jeffrey and Folkman, Judah and Gimbrone, Michael A., Jr. and Anderson, David J. (2001) Expression of EphrinB2 Identifies a Stable Genetic Difference Between Arterial and Venous Vascular Smooth Muscle as Well as Endothelial Cells, and Marks Subsets of Microvessels at Sites of Adult Neovascularization. Developmental Biology, 230 (2). pp. 139-150. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20150226-153358499

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Abstract

The transmembrane ligand ephrinB2 and its receptor tyrosine kinase EphB4 are molecular markers of embryonic arterial and venous endothelial cells, respectively, and are essential for angiogenesis. Here we show that expression of ephrinB2 persists in adult arteries where it extends into some of the smallest diameter microvessels, challenging the classical view that capillaries have neither arterial nor venous identity. EphrinB2 also identifies arterial microvessels in several settings of adult neovascularization, including tumor angiogenesis, contravening the dogma that tumor vessels arise exclusively from postcapillary venules. Unexpectedly, expression of ephrinB2 also defines a stable genetic difference between arterial and venous vascular smooth muscle cells. These observations argue for revisions of classical concepts of capillary identity and the topography of neovascularization. They also imply that ephrinB2 may be functionally important in neovascularization and in arterial smooth muscle, as well as in embryonic angiogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2000.9957DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160600999573PublisherArticle
Additional Information:© 2001 Academic Press. Received for publication August 11, 2000; Revised September 28, 2000; Accepted September 28, 2000; Published online January 19, 2001. We thank Shirley Pease for management of transgenic animals, Gabriele Mosconi for laboratory management, D. Panigrahy for assistance with tumor implantation, and Scott Gaspard and Hieu Phan for technical assistance. We also thank George Yancopoulos for calling our attention to the expression of ephrinB2 in the vaso vasorum. This work was supported in part by a grant from the American Heart Association to D.J.A. and the National Heart, Lung and Blood Institute (R37-HL51150 and P50-HL56985, to M.A.G.). S.-I.H. is the recipient of a Japan Heart Foundation Research Abroad Award. D.J.A. is an Investigator of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
American Heart AssociationUNSPECIFIED
National Heart, Lung and Blood Institute R37-HL51150
National Heart, Lung and Blood Institute P50-HL56985
Japan Heart Foundation Research Abroad AwardUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20150226-153358499
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150226-153358499
Official Citation:Shin, D., Garcia-Cardena, G., Hayashi, S.-I., Gerety, S., Asahara, T., Stavrakis, G., . . . Anderson, D. J. (2001). Expression of EphrinB2 Identifies a Stable Genetic Difference Between Arterial and Venous Vascular Smooth Muscle as Well as Endothelial Cells, and Marks Subsets of Microvessels at Sites of Adult Neovascularization. Developmental Biology, 230(2), 139-150. doi: http://dx.doi.org/10.1006/dbio.2000.9957
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55282
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:27 Feb 2015 20:15
Last Modified:03 Oct 2019 08:04

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