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Temporally Compartmentalized Expression of Ephrin-B2 during Renal Glomerular Development

Takahashi, Takamune and Takahashi, Keiko and Gerety, Sebastian and Wang, Hai and Anderson, David J. and Daniel, Thomas O. (2001) Temporally Compartmentalized Expression of Ephrin-B2 during Renal Glomerular Development. Journal of the American Society of Nephrology, 12 (12). pp. 2673-2682. ISSN 1046-6673. https://resolver.caltech.edu/CaltechAUTHORS:20150303-084726842

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Abstract

Glomerular development proceeds through the spatially ordered and sequential recruitment, proliferation, assembly, and differentiation of endothelial, mesangial, and epithelial progenitors. The molecular determinants of cell-cell recognition and targeting in this process have yet to be defined. The Eph/ephrin family of membrane receptors and counter-receptors are critical participants of developmental vascular assembly in extrarenal sites. Renal expression patterns of ephrin-B2 and EphB4 were investigated using mice expressing β-galactosidase under control of ephrin-B2 or EphB4 promoters. The earliest glomerular expression of ephrin-B2 was identified in a subset of differentiating comma-stage glomerular epithelial cells (podocyte progenitors) adjacent to the vascular cleft where endothelial progenitors are subsequently recruited. Epithelial ephrin-B2 expression was accompanied by expression in endothelial and mesangial cells as capillary assembly progressed. At or near completion of glomerular maturation, epithelial ephrin-B2 expression was extinguished, with persistence in glomerular endothelial cells. Throughout development, one of several ephrin-B2 receptors, EphB4, was persistently and exclusively expressed in endothelial cells of venous structures. The findings show sequential ephrin-B2 expression across glomerular lineages, first in a distinct subset of podocyte progenitors and subsequently in endothelial cells of the developing glomerulus. Given targeting functions for Eph/ephrin family proteins, the findings suggest that ephrin-B2 expression marks podocyte progenitors at the site of vascular cleft formation, where expression may establish an “address” to which endothelial and mesangial progenitors are recruited. Thus, the present results suggest that ephrin-B2 and EphB interactions play an important role in glomerular microvascular assembly.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jasn.asnjournals.org/content/12/12/2673PublisherArticle
Additional Information:© 2001 American Society of Nephrology. Received October 27, 2000; Accepted July 11, 2001. This work was supported by National Institutes of Health Grant R01-DK-47078 (T.O.D.). Analysis was performed in part through use of the VUMC Cell Imaging Resource supported by CA68485 and DK20593. The authors thank Raymond Harris and Matthew Breyer for support and advice, Dale Abrahamson for providing the antilaminin antibody, Steven Hebert for providing the anti-rTSC1 antibody, and John Schlueter and Amy Hsu for technical assistance.
Funders:
Funding AgencyGrant Number
NIHR01-DK-47078
NIHCA68485
NIHDK20593
Issue or Number:12
Record Number:CaltechAUTHORS:20150303-084726842
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150303-084726842
Official Citation:Takahashi, T., Takahashi, K., Gerety, S., Wang, H., Anderson, D. J., & Daniel, T. O. (2001). Temporally Compartmentalized Expression of Ephrin-B2 during Renal Glomerular Development. Journal of the American Society of Nephrology, 12(12), 2673-2682.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55451
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:03 Mar 2015 17:40
Last Modified:03 Oct 2019 08:05

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