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A single population of olfactory sensory neurons mediates an innate avoidance behaviour in Drosophila

Suh, Greg S. and Wong, Allan M. and Hergarden, Anne C. and Wang, Jing W. and Simon, Anne F. and Benzer, Seymour and Axel, Richard and Anderson, David J. (2004) A single population of olfactory sensory neurons mediates an innate avoidance behaviour in Drosophila. Nature, 431 (7010). pp. 854-859. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20150304-125624159

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Abstract

All animals exhibit innate behaviours in response to specific sensory stimuli that are likely to result from the activation of developmentally programmed neural circuits. Here we observe that Drosophila exhibit robust avoidance to odours released by stressed flies. Gas chromatography and mass spectrometry identifies one component of this ‘Drosophila stress odorant (dSO)’ as CO_2. CO_2 elicits avoidance behaviour, at levels as low as 0.1%. We used two-photon imaging with the Ca^(2+)-sensitive fluorescent protein G-CaMP to map the primary sensory neurons governing avoidance to CO_2. CO_2 activates only a single glomerulus in the antennal lobe, the V glomerulus; moreover, this glomerulus is not activated by any of 26 other odorants tested. Inhibition of synaptic transmission in sensory neurons that innervate the V glomerulus, using a temperature-sensitive Shibire gene (Shi^(ts))^1, blocks the avoidance response to CO_2. Inhibition of synaptic release in the vast majority of other olfactory receptor neurons has no effect on this behaviour. These data demonstrate that the activation of a single population of sensory neurons innervating one glomerulus is responsible for an innate avoidance behaviour in Drosophila.


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http://dx.doi.org/10.1038/nature02980DOIArticle
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http://www.nature.com/nature/journal/v431/n7010/suppinfo/nature02980.htmlPublisherSupporting Information
Additional Information:© 2004 Nature Publishing Group. Received 13 July; Accepted 1 September 2004; Published online 15 September 2004. We thank J.-S. Chang for technical assistance, L. Vosshall for providing Or83b-Gal4 and Or47b-Gal4 flies and for other unpublished information, D. Armstrong for Gal4 enhancer trap lines 103Y, 253Y, c747 and c761, T. Kitamoto for UAS-Shi^(ts) flies, U. Heberlein for the HU protocol and R. I. Wilson for discussion of unpublished data and comments on the manuscript. G.S.B.S. is a recipient of a National Research Service Award. A.C.H. is supported by a Howard Hughes Predoctoral fellowship. This work was supported by the HHMI (R.A. and D.J.A.) and by the NSF (S.B.). R.A. and D.J.A. are Investigators of the Howard Hughes Medical Institute.
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Funding AgencyGrant Number
NIH Predoctoral FellowshipUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:7010
Record Number:CaltechAUTHORS:20150304-125624159
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150304-125624159
Official Citation:Suh, G. S. B., Wong, A. M., Hergarden, A. C., Wang, J. W., Simon, A. F., Benzer, S., . . . Anderson, D. J. (2004). A single population of olfactory sensory neurons mediates an innate avoidance behaviour in Drosophila. [10.1038/nature02980]. Nature, 431(7010), 854-859. doi: http://www.nature.com/nature/journal/v431/n7010/suppinfo/nature02980_S1.html
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55519
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:05 Mar 2015 03:49
Last Modified:03 Oct 2019 08:06

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