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Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements

Coppock, Matthew B. and Farrow, Blake and Finch, Amethist S. and Lai, Bert and Sarkes, Deborah and Maciel, Jorge and Heath, James and Stratis-Cullum, Dimitra N. (2014) Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements. Abstracts of Papers of the American Chemical Society, 247 . 436-BIOT. ISSN 0065-7727 . http://resolver.caltech.edu/CaltechAUTHORS:20150312-110207059

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Abstract

Current biodetection assays that employ monoclonal antibodies as primary capture agents exhibit limited fieldability, shelf life, and performance due to batch-to-batch prodn. variability and restricted thermal stability. In order to improve upon the detection of biol. threats in fieldable assays and systems for the Army, we are investigating protein catalyzed capture (PCC) agents as drop-in replacements for the existing antibody technol. through iterative in situ click chem. The PCC agent oligopeptides are developed against known protein epitopes and can be mass produced using robotic methods. In this work, a PCC agent under development will be discussed. The performance, including affinity, selectivity, and stability of the capture agent technol., is analyzed by immunopptn., western blotting, and ELISA expts. The oligopeptide demonstrates superb selectivity coupled with high affinity through multi-ligand design, and improved thermal, chem., and biochem. stability due to non-natural amino acid PCC agent design.


Item Type:Article
ORCID:
AuthorORCID
Heath, James0000-0001-5356-4385
Additional Information:© 2014 American Chemical Society.
Record Number:CaltechAUTHORS:20150312-110207059
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150312-110207059
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55731
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Mar 2015 21:51
Last Modified:25 Apr 2017 04:07

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