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Identification and Characterization of HTLV-1 HBZ Post-Translational Modifications

Dissinger, Nathan and Shkriabai, Nikolozi and Hess, Sonja and Al-Saleem, Jacob and Kvaratskhelia, Mamuka and Green, Patrick L. (2014) Identification and Characterization of HTLV-1 HBZ Post-Translational Modifications. PLoS ONE, 9 (11). Art. No. e112762. ISSN 1932-6203. PMCID PMC4229220. http://resolver.caltech.edu/CaltechAUTHORS:20150313-112116963

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Abstract

Human T-cell leukemia virus type-1 (HTLV-1) is estimated to infect 15–25 million people worldwide, with several areas including southern Japan and the Caribbean basin being endemic. The virus is the etiological agent of debilitating and fatal diseases, for which there is currently no long-term cure. In the majority of cases of leukemia caused by HTLV-1, only a single viral gene, hbz, and its cognate protein, HBZ, are expressed and their importance is increasingly being recognized in the development of HTLV-1-associated disease. We hypothesized that HBZ, like other HTLV-1 proteins, has properties and functions regulated by post-translational modifications (PTMs) that affect specific signaling pathways important for disease development. To date, PTM of HBZ has not been described. We used an affinity-tagged protein and mass spectrometry method to identify seven modifications of HBZ for the first time. We examined how these PTMs affected the ability of HBZ to modulate several pathways, as measured using luciferase reporter assays. Herein, we report that none of the identified PTMs affected HBZ stability or its regulation of tested pathways.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0112762DOIArticle
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112762PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229220/PubMed CentralArticle
ORCID:
AuthorORCID
Hess, Sonja0000-0002-5904-9816
Additional Information:© 2014 Dissinger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received June 27, 2014; Accepted October 14, 2014; Published November 12, 2014. National Cancer Institute National Institutes of Health CA100730 to PLG (http://www.cancer.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funders:
Funding AgencyGrant Number
National Cancer InstituteCA100730
PubMed Central ID:PMC4229220
Record Number:CaltechAUTHORS:20150313-112116963
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150313-112116963
Official Citation:Dissinger N, Shkriabai N, Hess S, Al-Saleem J, Kvaratskhelia M, et al. (2014) Identification and Characterization of HTLV-1 HBZ Post-Translational Modifications. PLoS ONE 9(11): e112762. doi:10.1371/journal.pone.0112762
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55747
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:14 Mar 2015 01:04
Last Modified:14 Mar 2015 01:04

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