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A synthetic multicellular system for programmed pattern formation

Basu, Subhayu and Gerchman, Yoram and Collins, Cynthia H. and Arnold, Frances H. and Weiss, Ron (2005) A synthetic multicellular system for programmed pattern formation. Nature, 434 (7037). pp. 1130-1134. ISSN 0028-0836. doi:10.1038/nature03461. https://resolver.caltech.edu/CaltechAUTHORS:20150319-084633535

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Abstract

Pattern formation is a hallmark of coordinated cell behaviour in both single and multicellular organisms. It typically involves cell–cell communication and intracellular signal processing. Here we show a synthetic multicellular system in which genetically engineered ‘receiver’ cells are programmed to form ring-like patterns of differentiation based on chemical gradients of an acyl-homoserine lactone (AHL) signal that is synthesized by ‘sender’ cells. In receiver cells, ‘band-detect’ gene networks respond to user-defined ranges of AHL concentrations. By fusing different fluorescent proteins as outputs of network variants, an initially undifferentiated ‘lawn’ of receivers is engineered to form a bullseye pattern around a sender colony. Other patterns, such as ellipses and clovers, are achieved by placing senders in different configurations. Experimental and theoretical analyses reveal which kinetic parameters most significantly affect ring development over time. Construction and study of such synthetic multicellular systems can improve our quantitative understanding of naturally occurring developmental processes and may foster applications in tissue engineering, biomaterial fabrication and biosensing.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1038/nature03461DOIArticle
http://www.nature.com/nature/journal/v434/n7037/full/nature03461.htmlPublisherArticle
http://www.nature.com/nature/journal/v434/n7037/suppinfo/nature03461.htmlPublisherSupplementary Information
http://rdcu.be/cohcPublisherFree ReadCube access
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2005 Nature Publishing Group. Received 3 December 2004; accepted 14 February 2005. We thank D. Karig, S. Hooshangi, S. Thiberge, M.-T. Chen and S. Subramaniam for discussions or comments on the manuscript. This material is based on work supported by the Defense Advanced Research Projects Agency (DARPA).
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Issue or Number:7037
DOI:10.1038/nature03461
Record Number:CaltechAUTHORS:20150319-084633535
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150319-084633535
Official Citation:Basu, S., Gerchman, Y., Collins, C. H., Arnold, F. H., & Weiss, R. (2005). A synthetic multicellular system for programmed pattern formation. [10.1038/nature03461]. Nature, 434(7037), 1130-1134.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55914
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:24 Mar 2015 19:12
Last Modified:10 Nov 2021 20:52

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