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High numerical aperture Fourier ptychography: principle, implementation and characterization

Ou, Xiaoze and Horstmeyer, Roarke and Zheng, Guoan and Yang, Changhuei (2015) High numerical aperture Fourier ptychography: principle, implementation and characterization. Optics Express, 23 (3). pp. 3472-3491. ISSN 1094-4087. PMCID PMC5802253.

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Fourier ptychography (FP) utilizes illumination control and computational post-processing to increase the resolution of bright-field microscopes. In effect, FP extends the fixed numerical aperture (NA) of an objective lens to form a larger synthetic system NA. Here, we build an FP microscope (FPM) using a 40X 0.75NA objective lens to synthesize a system NA of 1.45. This system achieved a two-slit resolution of 335 nm at a wavelength of 632 nm. This resolution closely adheres to theoretical prediction and is comparable to the measured resolution (315 nm) associated with a standard, commercially available 1.25 NA oil immersion microscope. Our work indicates that Fourier ptychography is an attractive method to improve the resolution-versus-NA performance, increase the working distance, and enlarge the field-of-view of high-resolution bright-field microscopes by employing lower NA objectives.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle CentralArticle
Horstmeyer, Roarke0000-0002-2480-9141
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2015 Optical Society of America. Received 17 Nov 2014; revised 5 Jan 2015; accepted 5 Jan 2015; published 4 Feb 2015. This project was funded by the Caltech Innovation Initiative (CI2), Agency Award: 13520135; the National Institute of Health (NIH) Agency Award R01AI6226-01 and Clearbridge Biophotonics (Singapore). We thank Dr. Ana Rodriguez for providing the biological samples and input, Mr. Jinho Kim and Mr. Chao Han for fabricating the 2-hole and 2-slit targets, and Mr. Jaebum Chung for helpful discussions.
Funding AgencyGrant Number
Caltech Innovation Initiative (CI2)13520135
Clearbridge BiophotonicsUNSPECIFIED
Issue or Number:3
Classification Code:OCIS codes: (180.0180) Microscopy; (070.0070) Fourier optics and signal processing
PubMed Central ID:PMC5802253
Record Number:CaltechAUTHORS:20150323-111739047
Persistent URL:
Official Citation:X. Ou, R. Horstmeyer, G. Zheng, and C. Yang, "High numerical aperture Fourier ptychography: principle, implementation and characterization," Opt. Express 23, 3472-3491 (2015)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:55968
Deposited By: Tony Diaz
Deposited On:23 Mar 2015 19:43
Last Modified:09 Mar 2020 13:19

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