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Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells

Xue, Min and Wei, Wei and Su, Yapeng and Kim, Jungwoo and Shin, Young Shik and Mai, Wilson X. and Nathanson, David A. and Heath, James R. (2015) Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells. Journal of the American Chemical Society, 137 (12). pp. 4066-4069. ISSN 0002-7863. PMCID PMC4883658. http://resolver.caltech.edu/CaltechAUTHORS:20150324-093511464

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Abstract

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite–phosphoprotein correlation network.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/jacs.5b00944DOIArticle
http://pubs.acs.org/doi/abs/10.1021/jacs.5b00944PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/jacs.5b00944PublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883658/PubMed CentralArticle
ORCID:
AuthorORCID
Kim, Jungwoo0000-0002-5215-2044
Heath, James R.0000-0001-5356-4385
Additional Information:© 2015 American Chemical Society. Received: January 28, 2015. Publication Date (Web): March 19, 2015. This work was supported by the National Cancer Institute Grant No. 5U54 CA151819 (JRH PI), the Ben and Catherine Ivy Foundation, the Jean Perkins Foundation, and the Phelps Family Foundation. We acknowledge Professor Michael Phelps for helpful discussions regarding glucose assays. Author Contributions: M.X. and W.W. contributed equally. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
National Cancer Institute5U54 CA151819
Ben and Catherine Ivy FoundationUNSPECIFIED
Jean Perkins FoundationUNSPECIFIED
Phelps Family FoundationUNSPECIFIED
PubMed Central ID:PMC4883658
Record Number:CaltechAUTHORS:20150324-093511464
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150324-093511464
Official Citation:Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells Min Xue, Wei Wei, Yapeng Su, Jungwoo Kim, Young Shik Shin, Wilson X. Mai, David A. Nathanson, and James R. Heath Journal of the American Chemical Society 2015 137 (12), 4066-4069 DOI: 10.1021/jacs.5b00944
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56010
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Mar 2015 16:58
Last Modified:26 Oct 2017 19:38

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